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Recombinant Proteins & Enzymes

Recombinant BRD9 (130-259) protein

Aliases: PRO9856; LAVS3040
Catalog No: 31382 Format: 100 µg $395 Buy Now
Catalog No: 31882 Format: 1 mg $2,600 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant BRD9 (130-259) proteinContents

Recombinant BRD9 (130-259) protein was expressed in E. coli cells and is supplied in 25 mM Tris-HCl pH 8.0, 500 mM NaCl, 0.04% Triton X-100, 20% glycerol, 1mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant BRD9 (130-259) proteinBackground

Bromodomain-containing protein 9 (BRD9) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as ‘readers’ of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6) that function in the regulation of transcriptional activation and chromatin remodeling. There are five isoforms of BRD9 that are produced by alternative splicing.

for Recombinant BRD9 (130-259) proteinApplication Notes

Recombinant BRD9 (130-259) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant BRD9 (130-259) proteinProtein Details

The peptide corresponding to amino acids 130 - 259 that contains the bromodomain sequences of BRD9 (accession number NM_023924.4) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 20.9 kDa. The recombinant protein is >95% pure by SDS-PAGE.

 
BRD9 (130-259) Coomassie gel

Recombinant BRD9 (130-259) protein gel.
BRD9 (130-259) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.

BRD9 (130-259) HTRF activity assay

Recombinant BRD9 (130-259) activity assay.
3 µM histone peptide H4K5/8/12/16 (ac4) was incubated with BRD7 (130-259) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

for Recombinant BRD9 (130-259) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant BRD9 (130-259) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant BRD9 (130-259) protein
 

Data Thumbnails

BRD9 (130-259) Coomassie gel

BRD9 (130-259) Coomassie stained gel.
(Click image to enlarge and see details).

BRD9 (130-259) HTRF activity assay

BRD9 (130-259) HTRF activity assay.
(Click image to enlarge and see details).