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Recombinant Proteins & Enzymes

Recombinant PRMT5 protein

Catalog No: 31393 Format: 20 µg $425 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant PRMT5 proteinContents

Recombinant PRMT5 protein is supplied at a concentration of 0.3 µg/µl in 25 mM HEPES pH 7.5, 300 mM NaCl,10% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.

for Recombinant PRMT5 proteinBackground

PRMT5 (Protein Arginine Methyltransferase 5, also known as JBP1, Janus Binding Protein 1) is a type II arginine methyltransferase, a protein that transfers methyl groups to the arginine residues of substrate proteins. Arginine methylation is a common post-translational modification of histones and other cellular proteins. PRMT5 symmetrically dimethylates histone H2A Arg3, H3 Arg8 and H4 Arg3. It also methylates Piwi proteins which regulate small non-coding RNAs and snRNPs, regulating snRNP core particle assembly. PRMT5 functions as a co-repressor of transcription and is recruited by a number of DNA binding proteins and transcriptional repressor complexes. It is present both in the nucleus and the cytoplasm. It also interacts with COPR5, JAK2, PRDM1 and RPS10.

for Recombinant PRMT5 proteinApplication Notes

Recombinant PRMT5 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. PRMT5 is an active H3R8 and H4R3 methyltransferase.

HMT Assay Conditions: 0.5 µg Histone H4 (Cat. No. 31493) was incubated with different concentrations of PRMT5 protein in 30 µl reaction system including 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 µM SAM for 3 hours at room temperature. Activity was detected by Western Blot.

for Recombinant PRMT5 proteinProtein Details

Recombinant PRMT5 (accession number NP_006100.2) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 74.2 kDa. The recombinant protein is >70% pure by SDS-PAGE.

 
Recombinant PRMT5 protein gel.

Recombinant PRMT5 protein gel.
PRMT5 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

MW: 74 kDa

Purity: > 90%

PRMT5 activity assay

Recombinant PRMT5 activity assay.
0.5 µg of Histone H4 (Cat. No. 31493) was incubated with 0 µg (-), 0.15 µg (+), 0.3 µg (++), and 0.6 µg (+++) PRMT in 30 µl reaction volume containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 hr at room temp. 6 µl of reaction products were run on a 12.5% SDS-PAGE and detected by Western Blot using H4R3me2s antibody (Cat# 61187) at 1:1000. PRMT5 only was used as negative control.

for Recombinant PRMT5 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant PRMT5 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant PRMT5 protein
 

Data Thumbnails

Recombinant PRMT5 protein gel.

Recombinant PRMT5 protein gel.
(Click image to enlarge and see details).

PRMT5 activity assay

PRMT5 activity assay.
(Click image to enlarge and see details).