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Recombinant Proteins & Enzymes

Recombinant DNMT1 protein

Aliases: AIM, DNMT, MCMT, CXXC9, HSN1E, ADCADN
Catalog No: 31404 Format: 20 µg $395 Buy Now
Catalog No: 31800 Format: 1 mg $3,800 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant DNMT1 proteinContents

Full length recombinant DNMT1 protein is supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 1mM DTT and 0.2 mg/ml 3 × Flag peptide. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant DNMT1 proteinBackground

DNMT1 (DNA (Cytosine-5-)-Methyltransferase 1) proteins are involved in DNA methylation in which a methyl group is added to a cytosine residue on DNA, commonly at the C5 position of a CpG dinucleotide. Three families of DNMTs have been identified: DNMT1, DNMT2, and DNMT3 (comprised of DNMT3A and DNMT3B).

DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions. Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNA methylation is generally associated with transcriptional repression.

for Recombinant DNMT1 proteinApplication Notes

Recombinant DNMT1 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

DNMT Activity Assay Conditions: Assay was performed using DNMT Activity / Inhibition Assay, Active Motif Catalog No. 55006, for 1 hour at 37°C.

for Recombinant DNMT1 proteinProtein Details

Full length recombinant DNMT1 protein (accession number NP_001370.1) was expressed in Sf9 cells and contains an N-terminal 6×His-Tag and FLAG-Tag with a molecular weight of 188.2 kDa. The recombinant protein is >90% pure by SDS-PAGE.

 
DNMT1 protein Coomassie gel

Recombinant DNMT1 protein gel.
Recombinant DNMT1 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.

DNMT1 protein DNMT assay

Recombinant DNMT1 protein activity assay
Recombinant DNMT1 protein activity was measured using DNMT Activity / Inhibition Assay, Active Motif Catalog No. 55006, for 1 hour at 37°C

for Recombinant DNMT1 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant DNMT1 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant DNMT1 protein
 

Data Thumbnails

DNMT1 protein Coomassie gel

DNMT1 protein Coomassie stained gel.
(Click image to enlarge and see details).

DNMT1 protein DNMT assay

DNMT1 protein DNMT assay.
(Click image to enlarge and see details).