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Recombinant Proteins & Enzymes

Recombinant Tet2 (1129-2002) protein

Aliases: MDS, KIAA1546
Catalog No: 31418 Format: 10 µg $415 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant TET2 (1129-2002) proteinContents

Recombinant TET2 (1129-2002) protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant TET2 (1129-2002) proteinBackground

TET (Ten Eleven Translocation) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). 5hmC can be further oxidized into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for initiating the enzymatic deamination process leading to cytosine demethylation.

TET2 plays an important role in normal myelopoiesis. Somatic loss-of-function mutations in TET2 gene frequently occur in patients with myeloid malignancies. TET2 loss increased hematopoietic stem cell self-renewal and myeloid transformation. TET2 promotes histone O-GlcNAcylation during gene transcription in embryonic stem cells. In addition, TET2 can be recruited to chromatin by CXXC domain protein IDAX. IDAX downregulates TET2 protein by activating caspase pathway.

for Recombinant TET2 (1129-2002) proteinApplication Notes

Recombinant TET2 (1129-2002) protein is suitable for use in cytosine oxygenase assays, enzyme kinetics, inhibitor screening, and selectivity profiling.

TET Activity Assay Conditions: 250 nM of 30bp DNA oligos (sequence: 5'-CAGTAACTGTGGTC(mC)GGTAACTGACTTGCA-3', contains a 5mCpG site) was incubated with 100 nM recombinant TET2 (1129-2002) protein in buffer containing 50 mM HEPES, pH 8.0, 100 μM Fe(NH4)2(SO4)2, 2 mM ascorbate, 1 mM alpha-ketoglutarate, 1 mM ATP, 1 mM TCEP at 37C for 1 hr. Activity was detected by Dot-blot.

for Recombinant TET2 (1129-2002) proteinProtein Details

Recombinant TET2 (1129-2002) protein that includes amino acids 1129-2002 of human TET2 proteins (accession number NP_001120680.1) was expressed in Sf9 cells and contains an N-terminal 6×His-Tag and FLAG-Tag with a molecular weight of 102.7 kDa. The purity of the protein is >70% by SDS-PAGE.

 
Recombinant TET2 protein gel.

Recombinant TET2 (1129-2002) protein gel.
Recombinant TET2 (1129-2002) protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.

Recombinant TET2 protein activity assay

Recombinant TET2 (1129-2002) protein activity.
250 ng of 30 base oligo (containing 5mCpG) was incubated with 0.3125 µg, 0.625 µg or 1.25 µg TET2 in reaction buffer at 37°C for 1 hour. Sample was concentrated to 5 µl, and 1 µl was spotted onto nylon membrane. Anti-5hmC Ab (Cat#: 39769) was used to detect products.

for Recombinant TET2 (1129-2002) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant TET2 (1129-2002) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant Tet2 (1129-2002) protein
 

Data Thumbnails

Recombinant TET2 protein gel.

Recombinant TET2 protein gel, stained with Coomassie Blue.
(Click image to enlarge and see details).

Recombinant TET2 protein activity assay

Recombinant TET2 protein activity assay.
(Click image to enlarge and see details).