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Recombinant Proteins & Enzymes

Recombinant KMT2B (MLL2) protein

Aliases: ALR; KMS; KMT2D; MLL4; AAD10; KABUK1; TNRC21; CAGL114
Catalog No: 31420 Format: 20 μg $395 Buy Now
Catalog No: 31820 Format: 1 mg $3,200 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant KMT2B (MLL2) proteinContents

Recombinant KMT2B (MLL2) protein was expressed in E. coli cells and supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant KMT2B (MLL2) proteinBackground

KMT2B (MLL2) (Myeloid/lymphoid or mixed-lineage leukemia 2) is a Trithorax-group protein that function collectively to promote gene expression. KMT2B (MLL2) is a histone methyltransferase that methylates the Lysine 4 position of histone H3. In particular, the SET domain is a conserved C-terminal domain that characterizes proteins of the MLL (mixed-lineage leukemia) family. The SET domain is responsible for its histone methyltransferase activity which mediates chromatin modifications associated with epigenetic transcriptional activation. H3K4 methylation represents a specific epigenetic tag for transcriptional activation. KMT2B (MLL2) is part of a large protein complex called ASCOM, which has been shown to be a transcriptional regulator of the β-globin and estrogen receptor genes. Mutations in this gene have been shown to be a cause of Kabuki syndrome.

for Recombinant KMT2B (MLL2) proteinApplication Notes

Recombinant KMT2B (MLL2) protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

for Recombinant KMT2B (MLL2) proteinProtein Details

The peptide corresponding to amino acids 5355 - 5537 of the KMT2B (MLL2) protein (accession number NP_003473.3) was expressed in E. coli. The peptide contains the SET domain of MLL2 that is responsible for methyltransferase activity. Recombinant KMT2B (MLL2) protein contains an N-terminal GST-Tag with a molecular weight of 47.6 kDa. The recombinant protein is ≥ 80% pure by SDS-PAGE.

 
Recombinant KMT2B (MLL2) protein Coomassie gel

Recombinant KMT2B (MLL2) protein gel.
MLL2-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

Recombinant KMT2B (MLL2) protein binding assay

Recombinant KMT2B (MLL2) protein binding assay.
MLL2-SET was incubated with equimolar other 4 components of MLL2 complex (ASH2L, RBBP5, WDR5 and DPY30) for 2 hours 4 °C.
All the components were pulled down by GST beads. The sample was run on an 10% SDS-PAGE gel and stained with Coomassie Blue.

for Recombinant KMT2B (MLL2) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant KMT2B (MLL2) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant KMT2B (MLL2) protein
 

Data Thumbnails

Recombinant KMT2B (MLL2) protein Coomassie gel

Recombinant KMT2B (MLL2) protein Coomassie stained gel.
(Click image to enlarge and see details).

Recombinant KMT2B (MLL2) protein binding assay

Recombinant KMT2B (MLL2) protein binding assay.
(Click image to enlarge and see details).