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Recombinant Proteins & Enzymes

Recombinant EHMT2-SET (G9a) protein

Aliases: EHMT2; BAT8; GAT8; NG36; KMT1C; C6orf30
Catalog No: 31425 Format: 50 µg $395 Buy Now
Catalog No: 31825 Format: 1 mg $2,900 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant EHMT2-SET (G9a) proteinContents

Recombinant EHMT2-SET (G9a) protein expressed in E. coli and supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl and 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant EHMT2-SET (G9a) proteinBackground

EHMT2-SET (G9a), also known as Euchromatic Histone-lysine N-methyltransferase 2 (EHMT2), is a histone methyltransferase that specifically mono- and dimethylates Lys9 of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by initiating recruitment of HP1 proteins to methylated histones. EHMT2-SET (G9a) also mediates monomethylation of Lys56 of histone H3 (H3K56me1) in G1 phase and regulates DNA replication by promoting the interaction between histone H3 and PCNA. G9a also weakly methylates Lys27 of histone H3 (H3K27me). In addition, G9a is required for DNA methylation. However, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. G9a is most likely targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. G9a may also methylate histone H1. In addition to histone methyltransferase activity, G9a also methylates non-histone proteins. It has been shown to mediate dimethylation of Lys373 of p53 and also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.

for Recombinant EHMT2-SET (G9a) proteinApplication Notes

Recombinant EHMT2-SET (G9a) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

for Recombinant EHMT2-SET (G9a) proteinProtein Details

The peptide corresponding to amino acids 913 - 1193 of the EHMT2-SET (G9a) protein (accession number NP_006700.3) was expressed in E. coli. The peptide contains the SET domain of G9a that is responsible for methyltransferase activity. Recombinant G9a-SET contains N-terminal GST-Tag with an observed molecular weight of 58.7 kDa.

 
EHMT2-SET (G9a) Coomassie gel

Recombinant EHMT2-SET (G9a) protein gel.
G9a-SET protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

EHMT2-SET (G9a) activity assay

Recombinant EHMT2-SET (G9a) activity assay.
3.3 µM H3 (1-21) peptide was incubated with EHMT2-SET (G9a) protein in reaction buffer including 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 µM SAM for 3 hours at room temperature. Anti-H3K9me2 antibody is used to detect reaction product.

for Recombinant EHMT2-SET (G9a) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant EHMT2-SET (G9a) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant EHMT2-SET (G9a) protein
 

Data Thumbnails

EHMT2-SET (G9a) Coomassie gel

EHMT2-SET (G9a) Coomassie stained gel.
(Click image to enlarge and see details).

EHMT2-SET (G9a) activity assay

EHMT2-SET (G9a) activity assay.
(Click image to enlarge and see details).