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Recombinant Proteins & Enzymes

Recombinant LSD1 / KDM1A protein

Aliases: Lysine Demethylase 1A, Flavin-Containing Amine Oxidase Domain-Containing Protein 2, Lysine-Specific Histone Demethylase 1
Catalog No: 31426 Format: 20 μg $395 Buy Now
Catalog No: 31826 Format: 1 mg $3,500 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant LSD1 / KDM1A proteinContents

Full length recombinant KDM1A / LSD1 protein was expressed in Sf9 cells and is supplied in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant LSD1 / KDM1A proteinBackground

LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3 monomethyl Lys4 and Histone H3 dimethyl Lys4, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3 monomethyl Lys9 and Histone H3 dimethyl Lys9, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase.

for Recombinant LSD1 / KDM1A proteinApplication Notes

Recombinant LSD1 / KDM1A is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Catalytic Ability: > 600 turnovers / enzyme molecule.

Histone Demethylase Assay Conditions: 3.3 μM H3K4me2 (1-21aa) peptide was incubated with 5 nM KDM1A / LSD1 in reaction buffer containing 50 mM TrisCl, pH 8.0, 0.02% Triton X-100, 50 nM FAD for 1 hours at room temperature. MALDI-TOF were used for detection.

for Recombinant LSD1 / KDM1A proteinProtein Details

Recombinant LSD1 / KDM1A (accession number NP_055828) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with a molecular weight of 97.1 kDa. The recombinant protein is >90% pure by SDS-PAGE.

for Recombinant LSD1 / KDM1A proteinReferences

This protein is described in the following references:
Lan F et al. (2007) Nature 448:718-722
Forneris F et al. (2007) J Biol Chem. 281:35289-35295

 
LSD1 / KDM1A Coomassie gel

Recombinant LSD1 / KDM1A protein gel.
LSD1 / KDM1A protein was run on a 8% SDS-PAGE gel and stained with Coomassie blue.

LSD1 / KDM1A activity assay by HTRF

Recombinant LSD1 / KDM1A activity assay.
3.3 μM H3K4me2 (1-27aa) peptide was incubated with KDM1A / LSD1 in reaction buffer for 1 hour at room temperature. KDM1A / LSD1 enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K4me0) was measured using H3K4me0-specific antibody.

LSD1 / KDM1A activity assay by MALDI-TOF

Recombinant LSD1 / KDM1A activity assay.
3.3 uM H3K4me2 peptide was incubated with 5 nM LSD1 in reaction buffer for 1 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me2 peptide was used as negative control.

Catalytic Ability: >600 turnovers / enzyme molecule.

for Recombinant LSD1 / KDM1A proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant LSD1 / KDM1A proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant LSD1 / KDM1A protein
 

Data Thumbnails

LSD1 / KDM1A Coomassie gel

LSD1 / KDM1A Coomassie stained gel.
(Click image to enlarge and see details).

LSD1 / KDM1A activity assay by HTRF

LSD1 / KDM1A activity assay by HTRF.
(Click image to enlarge and see details).

LSD1 / KDM1A activity assay by MALDI-TOF

LSD1 / KDM1A activity assay by MALDI-TOF.
(Click image to enlarge and see details).