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Recombinant Proteins & Enzymes

Recombinant TAF1L (1392-1654) protein

Aliases: TAF2A2
Catalog No: 31436 Format: 100 μg $395 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant TAF1L (1392-1654) proteinContents

Recombinant TAF1L (1392-1654) protein is supplied in 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant TAF1L (1392-1654) proteinBackground

TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 210 kDa-like (TAF1L) is homologous to TAF1 and contains bromodomains for interaction with other proteins. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TAF1L is specifically expressed in the testis in male germ cells. It may act as a functional substitute for TAF1 during male meiosis when sex chromosomes are transcriptionally silenced.

for Recombinant TAF1L (1392-1654) proteinApplication Notes

Recombinant TAF1L (1392-1654) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant TAF1L (1392-1654) proteinProtein Details

The peptide corresponding to amino acids 1392-1654 that contains the bromodomain sequences of TAF1L (accession number NP_722516.1) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 36.747 kDa.

 
TAF1L (1392-1654) Coomassie gel

Recombinant TAF1L (1392-1654) protein gel.
TAF1L (1392-1654) protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

TAF1L (1392-1654) activity assay

Recombinant TAF1L (1392-1654) activity assay.
3.3 µM histone peptide H4K5/8/12/16 (4ac) was incubated with TAF1L (1392-1654) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

TAF1L (1392-1654) activity assay

Recombinant TAF1L (1392-1654) activity assay.
3.3 µM histone peptide H4K5/8/12/16 (4ac) was incubated with TAF1L (1392-1654) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

for Recombinant TAF1L (1392-1654) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant TAF1L (1392-1654) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant TAF1L (1392-1654) protein
 

Data Thumbnails

TAF1L (1392-1654) Coomassie gel

TAF1L (1392-1654) Coomassie stained gel.
(Click image to enlarge and see details).

TAF1L (1392-1654) activity assay

TAF1L (1392-1654) activity assay.
(Click image to enlarge and see details).

TAF1L (1392-1654) activity assay

TAF1L (1392-1654) activity assay.
(Click image to enlarge and see details).