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Recombinant Proteins & Enzymes

Recombinant SP140L (401-580) protein

Catalog No: 31437 Format: 100 μg $395 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant SP140L (401-580) proteinContents

Recombinant SP140L (401-580) protein is supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SP140L (401-580) proteinBackground

SP140 nuclear body protein-like (SP140L) is a protein with significant sequence similarity to SP140, a component of the nuclear body that may be involved in trafficking between the nucleus and the cytoplasm. The protein is expressed as four isoforms produced by alternative splicing, SP140L contains one HSR domain, one PHD-type zinc finger, a bromodomain and one SAND domain. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.

for Recombinant SP140L (401-580) proteinApplication Notes

Recombinant SP140L (401-580) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant SP140L (401-580) proteinProtein Details

The protein corresponding to amino acids 401-580 that contains the bromodomain sequence of human SP140L protein (accession number NP_612411.4) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with a molecular weight of 26.3 kDa. The purity of the protein is >90% by SDS-PAGE.

 
Recombinant SP140L (401-580) protein gel.

Recombinant SP140L (401-580) protein gel.
SP140L (401-580) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.
MW: 26.3 kDa
Purity: > 90%

HTRF Assay for Recombinant SP140L (401-580) protein activity.

HTRF Assay for Recombinant SP140L (401-580) protein activity.
3 µM histone peptide H3K14ac was incubated with SP140L (401-580) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

HTRF Assay for Recombinant SP140L (401-580) protein activity.

HTRF Assay for Recombinant SP140L (401-580) protein activity.
3 µM histone peptide H3K14ac was incubated with SP140L (401-580) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

for Recombinant SP140L (401-580) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SP140L (401-580) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant SP140L (401-580) protein
 

Data Thumbnails

Recombinant SP140L (401-580) protein gel.

Recombinant SP140L (401-580) protein gel, stained with Coomassie Blue.
(Click image to enlarge and see details).

HTRF Assay for Recombinant SP140L (401-580) protein activity.

HTRF Assay for Recombinant SP140L (401-580) protein activity.
(Click to enlarge and view details).

HTRF Assay for Recombinant SP140L (401-580) protein activity.

HTRF Assay for Recombinant SP140L (401-580) protein activity.
(Click to enlarge and view details).