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Recombinant Proteins & Enzymes

Recombinant TAF1 (1522-1656) protein

Aliases: TATA-Box Binding Protein Associated Factor 1, Cell Cycle Gene 1 Protein, TAF2A
Catalog No: 31439 Format: 100 μg $395 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant TAF1 (1522-1656) proteinContents

Recombinant TAF1 (1522-1656) protein is supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant TAF1 (1522-1656) proteinBackground

TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor (TAF1) is the largest component and core scaffold of the TFIID basal transcription factor complex. TAF1 contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. TAF1 also contains bromodomains for interaction with other proteins. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TAF1 has been shown to phosphorylate threonine 55 of p53 which leads to MDM2-mediated degradation of p53. TAF1 also phosphorylates GTF2A1 and GTF2F1 on serine residues. In addition, TAF1 possesses DNA-binding activity and is essential for progression of the G1 phase of the cell cycle.

for Recombinant TAF1 (1522-1656) proteinApplication Notes

Recombinant TAF1 (1522-1656) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant TAF1 (1522-1656) proteinProtein Details

The protein corresponding to amino acids 1522-1656 that contains the bromodomain sequence of TAF1 (accession number NP_004597.2) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 21.75 kDa. It shows binding specificity for acetylated H3K14.

 
Recombinant TAF1 (1522-1656) protein gel.

Recombinant TAF1 (1522-1656) protein gel.
TAF1 (1522-1656) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.
MW: 21.8 kDa
Purity: ≥ 98%

Recombinant TAF1 (1522-1656) protein activity assay.

Recombinant TAF1 (1522-1656) protein activity assay.
3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with Recombinant TAF1 (1522-1656) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.

Recombinant TAF1 (1522-1656) protein activity assay.

Recombinant TAF1 (1522-1656) protein activity assay.
3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with Recombinant TAF1 (1522-1656) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.

for Recombinant TAF1 (1522-1656) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant TAF1 (1522-1656) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant TAF1 (1522-1656) protein
 

Data Thumbnails

Recombinant TAF1 (1522-1656) protein gel.

Recombinant TAF1 (1522-1656) protein gel.
(Click image to enlarge and see details).

Recombinant TAF1 (1522-1656) protein activity assay.

Recombinant TAF1 (1522-1656) protein activity assay.
(Click image to enlarge and see details).

Recombinant TAF1 (1522-1656) protein activity assay.

Recombinant TAF1 (1522-1656) protein activity assay.
(Click image to enlarge and see details).