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Recombinant Proteins & Enzymes

Recombinant BRWD1 (1310-1430) protein

Aliases: N143; WDR9; C21orf107
Catalog No: 31440 Format: 100 μg $395 Buy Now
Catalog No: 31840 Format: 1 mg $2,600 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant BRWD1 (1310-1430) proteinContents

Recombinant BRWD1 (1310-1430) protein is supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant BRWD1 (1310-1430) proteinBackground

BRWD1 (Bromodomain and WD repeat domain containing 1) is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD) residues which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 2 bromodomains and multiple WD repeats. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. In mouse, this BRWD1 is a nuclear protein that has a polyglutamine-containing region that functions as a transcriptional activation domain. This domain associates with a component of the SWI/SNF chromatin remodeling complex and may function to regulate chromatin remodeling. BRWD1 plays a role in the regulation of cell morphology and cytoskeletal organization and is also required in the control of cell shape.

for Recombinant BRWD1 (1310-1430) proteinApplication Notes

Recombinant BRWD1 (1310-1430) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant BRWD1 (1310-1430) proteinProtein Details

The protein corresponding to amino acids 1310-1430 that contains the bromodomain sequence of human BRWD1 protein (accession number NP_061836.2) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with a molecular weight of 18.97 kDa. The purity of the protein is >92% by SDS-PAGE.

 
Recombinant BRWD1 (1310-1430) protein gel.

Recombinant BRWD1 (1310-1430) protein gel.
BRWD1 (1310-1430) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.
MW: 19 kDa
Purity: > 92%

HTRF Assay for Recombinant BRWD1 (1310-1430) protein activity.

HTRF assay for BRWD1 (1310-1430) activity assay.
3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRWD1 (1310-1430) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.

HTRF Assay for Recombinant BRWD1 (1310-1430) protein activity.

HTRF Assay for Recombinant BRWD1 (1310-1430) protein activity.
3 µM histone peptide H4K5/8/12/16 (4Ac) was incubated with BRWD1 (1310-1430) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products.

for Recombinant BRWD1 (1310-1430) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant BRWD1 (1310-1430) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant BRWD1 (1310-1430) protein
 

Data Thumbnails

Recombinant BRWD1 (1310-1430) protein gel.

Recombinant BRWD1 (1310-1430) protein gel, stained with Coomassie Blue.
(Click image to enlarge and see details).

HTRF Assay for Recombinant BRWD1 (1310-1430) protein activity.

HTRF Assay for Recombinant BRWD1 (1310-1430) protein activity
(Click image to enlarge and see details).

HTRF Assay for Recombinant BRWD1 (1310-1430) protein activity.

HTRF Assay for Recombinant BRWD1 (1310-1430) protein activity
(Click image to enlarge and see details).