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Recombinant Proteins & Enzymes

Recombinant ASH1L (2407-2579) protein

Aliases: ASH1; KMT2H; ASH1L1
Catalog No: 31445 Format: 100 μg $395 Buy Now
Catalog No: 31845 Format: 1 mg $2,600 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant ASH1L (2407-2579) proteinContents

Recombinant ASH1L (2407-2579) protein was expressed in E. coli cells and is supplied in 25 mM Tris pH 7.4, 150 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant ASH1L (2407-2579) proteinBackground

ASH1L is a member of the Trithorax group of transcriptional activators. The protein contains four AT hooks, a SET domain, a PHD-finger motif, and a bromodomain. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. ASH1L is localized to the nucleus and also to cell-cell tight junctions where it is hypothesized to function in adhesion-mediated signaling. ASH1L functions in methylation of H3K4 and regulation of expression of HOX genes and may possess methyltransferase activity directed at H3K36. ASH1L has also been implicated in facioscapulohumeral muscular dystrophy.

for Recombinant ASH1L (2407-2579) proteinApplication Notes

Recombinant ASH1L (2407-2579) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant ASH1L (2407-2579) proteinProtein Details

The peptide corresponding to amino acids 2407-2579 that contains the bromodomain sequences of ASH1L (accession number NP_060959.2) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 25.6 kDa.

 
ASH1L (2407-2579) Coomassie gel

Recombinant ASH1L (2407-2579) protein gel.
ASH1L (2407-2579) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.

ASH1L (2407-2579) activity assay

Recombinant ASH1L (2407-2579) activity assay.
3.3 µM histone peptide H4K5/8/12/16(4ac) was incubated with ASH1L (2407-2579) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

for Recombinant ASH1L (2407-2579) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant ASH1L (2407-2579) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant ASH1L (2407-2579) protein
 

Data Thumbnails

ASH1L (2407-2579) Coomassie gel

ASH1L (2407-2579) Coomassie stained gel.
(Click image to enlarge and see details).

ASH1L (2407-2579) activity assay

ASH1L (2407-2579) activity assay.
(Click image to enlarge and see details).