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Recombinant Proteins & Enzymes

Recombinant BRD4 (333-460) protein

Aliases: CAP; MCAP; HUNK1; HUNKI
Catalog No: 31446 Format: 100 μg $395 Buy Now
Catalog No: 31846 Format: 1 mg $2,600 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant BRD4 (333-460) proteinContents

Recombinant BRD4 (333-460) protein expressed in E. coli and supplied in 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant BRD4 (333-460) proteinBackground

BRD4 (Bromodomain-containing protein 4) belongs to the BET subclass of proteins, which are characterized by two N-terminal bromodomains and one ET (Extra Terminal) domain. BRDs associate with chromatin through their bromodomains that recognize acetylated histone lysine residues. Bromodomains function as ‘readers’ of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. The ET domain functions as a protein binding motif and exerts atypical serine-kinase activity. The BET family consists of at least four members in mouse and human, BRD2 (also referred to as FSRG1, RING3), BRD3 (FSRG2, ORFX), BRD4 (FSRG4, MCAP/HUNK1), and BRDT (FSRG3, BRD6). BRD proteins are related to the female Sterile Homeotic protein gene in Drosophila, a gene required maternally for proper expression of other homeotic genes, such as Ubx, which is involved in pattern formation. BRD4 has been identified recently as a therapeutic target in many cancers, including acute myeloid leukemia, multiple myeloma, Burkitt’s lymphoma, NUT midline carcinoma, colon cancer, and breast cancer. BRD4 regulates the transcription of oncogenes, HIV, and human papilloma virus (HPV). It has been shown to bind and phosphorylate RNA pol II, which implicates its involvement in the regulation of eukaryotic transcription.

for Recombinant BRD4 (333-460) proteinApplication Notes

Recombinant BRD4 (333-460) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant BRD4 (333-460) proteinProtein Details

The peptide corresponding to amino acids 333-460 that contains the bromodomain sequences of BRD4 (accession number NP_490597.1) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 20.9 kDa. The recombinant protein is >87% pure by SDS-PAGE.

 
BRD4 (333-460) Coomassie gel

Recombinant BRD4 (333-460) protein gel.
BRD4 (333-460) protein was run on an SDS-PAGE gel and stained with Coomassie blue.

BRD4 (333-460) HTRF activity assay

Recombinant BRD4 (333-460) HTRF activity assay
3.3 uM histone peptide H4K5/8/12/16(4Ac) was incubated with BRD4 (333-460) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-Flag antibody was used to detect reaction products

for Recombinant BRD4 (333-460) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant BRD4 (333-460) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant BRD4 (333-460) protein
 

Data Thumbnails

BRD4 (333-460) Coomassie gel

BRD4 (333-460) Coomassie stained gel.
(Click image to enlarge and see details).

BRD4 (333-460) HTRF activity assay

BRD4 (333-460) HTRF activity assay.
(Click image to enlarge and see details).