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Recombinant Proteins & Enzymes

Recombinant SP140 (688-862) protein

Aliases: LYSP100; LYSP100-A; LYSP100-B
Catalog No: 31448 Format: 100 μg $395 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant SP140 (688-862) proteinContents

Recombinant SP140 (688-862) protein was expressed in E. coli cells and is supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 0.04% Triton X-100, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SP140 (688-862) proteinBackground

SP140 nuclear body protein (SP140) is a component of the nuclear body that may be involved in trafficking between the nucleus and the cytoplasm. SP140 contains one HSR domain, one PHD-type zinc finger, a bromodomain and one SAND domain. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. SP140 may be involved in the pathogenesis of acute promyelocytic leukemia and viral infection.

for Recombinant SP140 (688-862) proteinApplication Notes

Recombinant SP140 (688-862) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant SP140 (688-862) proteinProtein Details

The peptide corresponding to amino acids 688-862 that contains the bromodomain sequences of SP140 (accession number NP_009168.4) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 26.98 kDa. The recombinant protein is ≥ 90% pure by SDS-PAGE.

 
Recombinant SP140 (688-862) protein gel.

Recombinant SP140 (688-862) protein gel.
SP140 (688-862) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.
MW: 26.9 kDa
Purity: > 90%

HTRF Assay for Recombinant SP140 (688-862) protein activity.

HTRF Assay for Recombinant SP140 (688-862) protein activity.
3 µM histone peptide H3K14ac was incubated with SP140 (688-862) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

HTRF Assay for Recombinant SP140 (688-862) protein activity.

HTRF Assay for Recombinant SP140 (688-862) protein activity.
3 µM histone peptide H3K14ac was incubated with SP140 (688-862) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

for Recombinant SP140 (688-862) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SP140 (688-862) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant SP140 (688-862) protein
 

Data Thumbnails

Recombinant SP140 (688-862) protein gel.

Recombinant SP140 (688-862) protein gel, stained with Coomassie Blue.
(Click image to enlarge and see details).

HTRF Assay for Recombinant SP140 (688-862) protein activity.

HTRF Assay for Recombinant SP140 (688-862) protein activity.
(Click image to enlarge and see details).

HTRF Assay for Recombinant SP140 (688-862) protein activity.

HTRF Assay for Recombinant SP140 (688-862) protein activity.
(Click image to enlarge and see details).