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Recombinant Proteins & Enzymes

Recombinant SMARCA2 / BRM (1367-1511) protein

Aliases: BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a
Catalog No: 31449 Format: 100 μg $395 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant SMARCA2 / BRM (1367-1511) proteinContents

Recombinant SMARCA2/BRM (1367-1511) protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SMARCA2 / BRM (1367-1511) proteinBackground

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2), also known as BRM, is a member of the SWI/SNF family of proteins and is similar to the Brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA2 contains bromodomains for interaction with other proteins. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR). SMARCA1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). SMARCA2 plays a pivotal role in the regulation of the switch in subunit composition of the npBAF and nBAF complexes as cells transition from proliferating neural stem/progenitor cells to post-mitotic neurons during neural development.

for Recombinant SMARCA2 / BRM (1367-1511) proteinApplication Notes

Recombinant SMARCA2 / BRM (1367-1511) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant SMARCA2 / BRM (1367-1511) proteinProtein Details

The peptide corresponding to amino acids 1367-1511 that contains the bromodomain sequences of SMARCA2 / BRM (accession number NP_003061.3) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with an observed molecular weight of 22.96 kDa. It shows binding specificity for acetylated H3K9, H3K14, H3K9/14, H4K8, H4K12, H4K16 and H4K5/8/12/16.

 
SMARCA2 / BRM (1367-1511) Coomassie gel

Recombinant SMARCA2 / BRM (1367-1511) protein gel.
SMARCA2 / BRM (1367-1511) protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

HTRF Assay for SMARCA2 / BRM (1367-1511) activity.

HTRF Assay for Recombinant SMARCA2 / BRM (1367-1511) activity.
3.3 µM histone peptide H4K5/8/12/16 (ac4) was incubated with SMARCCA2/BRM (1367-1511) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature.
Anti-FLAG antibody was used to detect reaction products.

HTRF Assay for SMARCA2 / BRM (1367-1511) activity.

HTRF Assay for Recombinant SMARCA2 / BRM (1367-1511) activity.
3.3 µM histone peptide H4K5/8/12/16 (ac4) was incubated with SMARCCA2/BRM (1367-1511) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature.
Anti-FLAG antibody was used to detect reaction products.

for Recombinant SMARCA2 / BRM (1367-1511) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SMARCA2 / BRM (1367-1511) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant SMARCA2 / BRM (1367-1511) protein
 

Data Thumbnails

SMARCA2 / BRM (1367-1511) Coomassie gel

SMARCA2 / BRM (1367-1511) Coomassie stained gel.
(Click image to enlarge and see details).

HTRF Assay for SMARCA2 / BRM (1367-1511) activity.

HTRF Assay for SMARCA2 / BRM (1367-1511) activity.
(Click image to enlarge and see details).

HTRF Assay for SMARCA2 / BRM (1367-1511) activity.

HTRF Assay for SMARCA2 / BRM (1367-1511) activity.
(Click image to enlarge and see details).