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Recombinant Proteins & Enzymes

Recombinant TAF1L (1402-1521) protein

Aliases: TAF2A2
Catalog No: 31451 Format: 100 μg $395 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant TAF1L (1402-1521) proteinContents

Recombinant TAF1L (1402-1521) protein is supplied in 25 mM Tris pH 8.0, 500 mM NaCl, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant TAF1L (1402-1521) proteinBackground

TAF1 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 210 kDa-like (TAF1L), is homologous to TAF1 and contains bromodomains for interaction with other proteins. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. TAF1L is specifically expressed in the testis in male germ cells. It may act as a functional substitute for TAF1 during male meiosis when sex chromosomes are transcriptionally silenced.

for Recombinant TAF1L (1402-1521) proteinApplication Notes

Recombinant TAF1L (1402-1521) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

for Recombinant TAF1L (1402-1521) proteinProtein Details

Recombinant TAF1L (1402-1521) protein that includes amino acids 1402-1521 of human TAF1L protein (accession number NP_722516.1) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with a molecular weight of 20.08 kDa. The purity of the protein is > 92% by SDS-PAGE.

 
Recombinant TAF1L (1402-1521) protein gel.

Recombinant TAF1L (1402-1521) protein gel.
TAF1L (1402-1521) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.
MW: 20.1 kDa
Purity: > 92%

HTRF Assay for TAF1L (1402-1521) protein activity.

HTRF Assay for Recombinant TAF1L (1402-1521) protein activity.
3 µM histone peptide H4K5/8/12/16(4ac) was incubated with TAF1L (1402-1521) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

HTRF Assay for TAF1L (1402-1521) protein activity.

HTRF Assay for Recombinant TAF1L (1402-1521) protein activity.
3 µM histone peptide H4K5/8/12/16(4ac) was incubated with TAF1L (1402-1521) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-FLAG antibody was used to detect reaction products.

for Recombinant TAF1L (1402-1521) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant TAF1L (1402-1521) proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant TAF1L (1402-1521) protein
 

Data Thumbnails

Recombinant TAF1L (1402-1521) protein gel.

Recombinant TAF1L (1402-1521) protein gel, stained with Coomassie Blue.
(Click image to enlarge and see details).

HTRF Assay for TAF1L (1402-1521) protein activity.

HTRF Assay for TAF1L (1402-1521) protein activity.
(Click image to enlarge and see details).

HTRF Assay for TAF1L (1402-1521) protein activity.

HTRF Assay for TAF1L (1402-1521) protein activity.
(Click image to enlarge and see details).