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Recombinant Proteins & Enzymes

Recombinant FBXL10 / KDM2B protein

Aliases: CXXC2; Fbl10; PCCX2; JHDM1B
Catalog No: 31455 Format: 20 µg $395 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant FBXL10 / KDM2B proteinContents

Full length recombinant FBXL10 / KDM2B protein expressed in Sf9 cells and is supplied in 25 mM HEPES-NaOH, pH 7.5, 300 mM NaCl, 5% glycerol and 0.2 mg/ml 3x Flag peptide. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant FBXL10 / KDM2B proteinBackground

KDM2B (lysine (K)-specific demethylase 2B), also known as FBXL10 (F-box and leucine-rich repeat protein 10) is a histone demethylase that preferentially demethylates trimethylated lysine 4 (K4me3) and dimethylated lysine 36 (K36me2) of histone H3. KDM2B displays weak or no activity for mono- and trimethylated H3K36. KDM2B preferentially binds the transcribed region of ribosomal RNA and represses the transcription of ribosomal RNA genes which results in inhibition of cell growth and proliferation. KDM2B may also serve as a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

for Recombinant FBXL10 / KDM2B proteinApplication Notes

Recombinant FBXL10 / KDM2B is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Histone Demethylase Assay Conditions: 50 mM HEPES pH 7.5, 0.02% Triton X100, 100 μM 2OG, 100 μM Ascorbate, 50 μM (NH4)2Fe(SO4)2·6H2O, 1 mM TCEP, 300 nM Recombinant FBXL10 / KDM2B protein, and 3.3 μM H3K36me2 peptide at 2 hours at room temperature. MALDI-TOF was used for detection.

for Recombinant FBXL10 / KDM2B proteinProtein Details

Recombinant FBXL10 / KDM2B (accession number NP_115979.3) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 155.9 kDa. The recombinant protein is >75% pure by SDS-PAGE.

 
FBXL10 / KDM2B Coomassie gel

Recombinant FBXL10 / KDM2B protein gel.
FBXL10 / KDM2B protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.

FBXL10 / KDM2B activity assay

FBXL10 / KDM2B activity assay.
Recombinant FBXL10 / KDM2B activity measured by incubating 300 nM FB XL10/KDM2B protein with 3.3 µM H3K36me2 peptide in reaction buffer for 2 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 µM H3K36me2 peptide was used as a negative control.

for Recombinant FBXL10 / KDM2B proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant FBXL10 / KDM2B proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant FBXL10 / KDM2B protein
 

Data Thumbnails

FBXL10 / KDM2B Coomassie gel

FBXL10 / KDM2B Coomassie stained gel.
(Click image to enlarge and see details).

FBXL10 / KDM2B activity assay

FBXL10 / KDM2B activity assay.
(Click image to enlarge and see details).