Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Recombinant Proteins & Enzymes

Recombinant KDM1B / LSD2 protein

Aliases: Lysine Demethylase 1B, Flavin-Containing Amine Oxidase Domain-Containing Protein 1
Catalog No: 31479 Format: 20 µg $395 Buy Now
Catalog No: 31879 Format: 1 mg $3,500 Buy Now
Expressed In: Baculovirus Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant KDM1B / LSD2 proteinContents

Full length recombinant KDM1B / LSD2 protein containing an N-terminal GST-Tag expressed in Sf9 cells and supplied in 25 mM HEPES, pH 7.5, 120 mM NaCl 20 mM GSH and 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant KDM1B / LSD2 proteinBackground

KDM1B / LSD2 (Lysine (K)-Specific Demethylase 1B / Lysine-Specific Histone Demethylase 2) is a histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4 (by similarity).

for Recombinant KDM1B / LSD2 proteinApplication Notes

Recombinant KDM1B / LSD2 protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Specific Activity: H3K4me2 and H3K4me1 demethylase.

Catalytic Ability: > 600 turnovers / enzyme molecule

Histone Demethylase Assay Conditions: 3.3 μM H3K4me2 (1-27aa) peptide was incubated with different concentrations of KDM1B / LSD2 in reaction buffer containing 50 mM TrisCl, pH 8.0, 0.02% Triton X-100, 50 nM FAD for 1 hours at room temperature. HTRF and MALDI-TOF were used for detection.

for Recombinant KDM1B / LSD2 proteinProtein Details

Full-length Recombinant KDM1B / LSD2 (accession number XM_005248926.1) was expressed in was expressed in Sf9 cells and contains an N-terminal GST-Tag with an observed molecular weight of 116 kDa. The recombinant protein is >92% pure by SDS-PAGE.

 
Recombinant KDM1B / LSD2 Coomassie gel

Recombinant KDM1B / LSD2 protein gel.
Recombinant KDM1B / LSD2 run on an 8% SDS-PAGE gel and stained with Coomassie blue.

KDM1B / LSD2 activity assay

Recombinant KDM1B / LSD2 protein activity assay.
3.3 uM H3K4me2 (1-21aa) peptide was incubated with 5 nM LSD2 in reaction buffer for 2 hours at room temperature. The reaction product was detected by MALDI-TOF. Single 3.3 uM H3K4me2 peptide was used as negative control.

Catalytic Ability: > 600 turnovers/ enzyme molecule

KDM1B / LSD2 activity assay

Recombinant KDM1B / LSD2 protein activity assay.
3.3 μM H3K4me2 (1-27aa) peptide was incubated with KDM1B / LSD2 in reaction buffer for 1 hour at room temperature. KDM1B / LSD2 enzyme was used in a HTRF assay to determine enzyme linearity. Demethylated peptide (H3K4me0) was measured using H3K4me0-specific antibody.

for Recombinant KDM1B / LSD2 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant KDM1B / LSD2 proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant KDM1B / LSD2 protein
 

Data Thumbnails

Recombinant KDM1B / LSD2 Coomassie gel

Recombinant KDM1B / LSD2 Coomassie stained gel.
(Click image to enlarge and see details).

KDM1B / LSD2 activity assay

KDM1B / LSD2 activity assay.
(Click image to enlarge and see details).

KDM1B / LSD2 activity assay

KDM1B / LSD2 activity assay.
(Click image to enlarge and see details).