Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Recombinant Proteins & Enzymes

Recombinant SETD7 protein

Aliases: KMT7, SET7, SET9, SET7/9
Catalog No: 31496 Format: 20 µg $395 Buy Now
Expressed In: Baculovirus Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant SETD7 proteinContents

Full length recombinant SETD7 protein was expressed in Sf9 cells and is supplied in 25 mM Hepes pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SETD7 proteinBackground

SETD7 (SET Domain Containing (Lysine Methyltransferase) 7) is a histone methyltransferase that specifically monomethylates ‘Lys-4’ of histone H3. H3 ‘Lys-4’ methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates ‘Lys-189’ of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates ‘Lys-372’ of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.

for Recombinant SETD7 proteinApplication Notes

Recombinant SETD7 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

HMT Assay Conditions: 1 μM H3K4me0 (1-21aa) peptide was incubated with different concentrations of recombinant SETD7 protein in reaction buffer containing 50 mM TrisCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 100 μM SAM for 2 hours at room temperature. Activity was detected by HTRF and Fluorography.

for Recombinant SETD7 proteinProtein Details

Recombinant SETD7 protein (accession number NP_085151.1) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with a molecular weight of 41.7 kDa. The recombinant protein is >80% pure by SDS-PAGE.

 
SETD7 protein Coomassie gel

Recombinant SETD7 protein gel.
SETD7 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.

SETD7 protein HTRF activity assay

Recombinant SETD7 protein HTRF activity assay.
1 μM H3K4me0 (1-21aa) peptide was incubated with SETD7 in reaction buffer for 2 hour at room temperature. SETD7 enzyme was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me1) was measured using H3K4me1-specific antibody.

SETD7 protein activity assay

Recombinant SETD7 protein activity assay.
2 ug Calf Thymus Octamers was incubated with 0.5 ug SETD7 protein in reaction buffer containing radioactive 3H-SAM for 2 hour at room temperature. Activity was detected by fluorography.

for Recombinant SETD7 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SETD7 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant SETD7 protein
 

Data Thumbnails

SETD7 protein Coomassie gel

SETD7 protein Coomassie gel.
(Click image to enlarge and see details).

SETD7 protein HTRF activity assay

SETD7 protein HTRFactivity assay.
(Click image to enlarge and see details).

SETD7 protein activity assay

SETD7 protein activity assay.
(Click image to enlarge and see details).