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Recombinant Proteins & Enzymes

Recombinant AKT1 protein

Aliases: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA
Catalog No: 31511 Format: 10 µg $395 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant AKT1 proteinContents

Full length recombinant AKT1 protein expressed in Sf9 cells and supplied in 50 mM HEPES pH 7.5, 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant AKT1 proteinBackground

AKT1 is a serine-threonine protein kinase that is activated by Platelet-Derived Growth Factor (PDGF) and PI 3-kinase signaling pathways. AKT has been shown to be a critical mediator of survival and apoptosis signaling events. Activation of AKT1 involves (and requires) phosphorylation of serine 473. AKT is also referred to as Protein Kinase B (PKB) and RAC.

for Recombinant AKT1 proteinApplication Notes

Recombinant AKT1 is suitable for kinase assays. The molecular weight of the fusion protein is ~ 81 kDa.

Specific activity: 55 pmol/μg×min ATP-KM: 5.0 μM

for Recombinant AKT1 proteinProtein Details

Recombinant AKT11 (accession number NP_005154.2) activated with PDK1, was expressed in Sf9 cells is an N-terminal GST-HIS6 fusion protein with a 3C cleavage site.

 
AKT1 protein Coomassie gel

Recombinant AKT1 protein gel.
AKT1 protein was run on an SDS-PAGE gel and stained with Coomassie blue.

for Recombinant AKT1 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant AKT1 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant AKT1 protein
 

Data Thumbnails

AKT1 protein Coomassie gel

AKT1 protein Coomassie gel.
(Click image to enlarge and see details).