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Recombinant Proteins & Enzymes

Recombinant JARID1B / KDM5B (2-751) protein

Aliases: Lysine Demethylase 5B, Cancer/Testis Antigen 31, Retinoblastoma-Binding Protein 2 Homolog 1, Histone Demethylase JARID1B
Catalog No: 31518 Format: 20 µg $395 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant JARID1B / KDM5B (2-751) proteinContents

Recombinant JARID1B / KDM5B (2-751) protein was expressed in Sf9 cells and is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant JARID1B / KDM5B (2-751) proteinBackground

Lysine (K)-specific demethylase 5B (KDM5B), also known as Jumonji, AT rich interactive domain 1B (JARID1B), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5B demethylates trimethylated, dimethylated and monomethylated H3K4. It does not demethylate histone H3K9 or H3K27. KDM5B functions as a transcriptional corepressor for FOXG1B and PAX9. KDM5B induces the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, it may act as a tumor suppressor for melanoma.

for Recombinant JARID1B / KDM5B (2-751) proteinApplication Notes

Recombinant JARID1B / KDM5B (2-751) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiing.

HDM Activity Assay Conditions: 3 µM H3K4me3 (1-21aa) peptide was incubated with KDM5B (2-751) protein in reaction buffer including 50 mM HEPES pH 7.5, 0.02% Triton X-100, 100 µM 2-OG, 100 µM ascorbate, 50 µM (NH4)2Fe(SO4)2·6H2O for 1 hour at room temperature. HTRF assay and MALDI-TOF was used for activity detection.

for Recombinant JARID1B / KDM5B (2-751) proteinProtein Details

Recombinant JARID1B / KDM5B (2-751) (accession number NP_006609) was expressed in Sf9 cells and contains an N-terminal FLAG-Tag with an observed molecular weight of 100 kDa. The recombinant protein is ≥ 80% pure by SDS- PAGE.

 
Recombinant JARID1B / KDM5B (2-751) SDS-PAGE Gel

Recombinant JARID1B / KDM5B (2-751) was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.
MW: 100 kDa
Purity: >80%

HTRF assay for JARID1B / KDM5B (2-751) activity

HTRF assay for JARID1B /KDM5B (2-751) activity

3 µM H3K4me3 (1-21 aa) peptide was incubated with KDM5B (2-751) protein in reaction buffer for 1 hour at room temperature.
Anti-H3K4me2-d2 antibody was used to detect reaction product.

MALDI-TOF for JARID1B / KDM5B (2-751) activity

MALDI-TOF for JARID1B / KDM5B (2-751) activity.

3.3 µM H3K4me3 (1-21 aa) peptide was incubated with 100 nM or 200 nM JARID1B / KDM5B (2-751) protein in reaction buffer for 2 hours at room temperature. MALDI-TOF was used for detection.

Catalytic Ability: > 60 turnovers/ enzyme molecule

for Recombinant JARID1B / KDM5B (2-751) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant JARID1B / KDM5B (2-751) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant JARID1B / KDM5B (2-751) protein
 

Data Thumbnails

Recombinant JARID1B / KDM5B (2-751) SDS-PAGE Gel

Recombinant JARID1B / KDM5B (2-751) was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.
(Click image to enlarge and see details).

HTRF assay for JARID1B / KDM5B (2-751) activity

HTRF assay for JARID1B / KDM5B (2-751) activity.
(Click image to enlarge and see details)

MALDI-TOF for JARID1B / KDM5B (2-751) activity

MALDI-TOF for JAIRD1B / KDM5B (2-751) activity.
(Click image to enlarge and see details)