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Recombinant Proteins & Enzymes

Recombinant JMJD3 / KDM6B (1043-1682) protein

Aliases: Jumonji Domain Containing 3 Histone Lysine Demethylase, KDM6B, Lysine Demethylase 6B
Catalog No: 31519 Format: 20 µg $395 Buy Now
Catalog No: 31919 Format: 1 mg $3,500 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant JMJD3 / KDM6B (1043-1682) proteinContents

Recombinant JMJD3 / KMD6B (1043-1682) protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant JMJD3 / KDM6B (1043-1682) proteinBackground

JMJD3 / KMD6B (Jumonji domain-containing protein 3 lysine (K)-specific demethylase 6B) is a histone demethylase that specifically demethylates ‘Lys-27’ of histone H3, thereby, playing a central role in histone code. JMJD3 demethylates trimethylated and dimethylated H3 ‘Lys-27’. It plays a central role in regulation of posterior development by regulating HOX gene expression. JMJD3 / KDM6B is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. 

for Recombinant JMJD3 / KDM6B (1043-1682) proteinApplication Notes

Recombinant JMJD3 / KDM6B (1043-1682) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

HDM Activity Assay Conditions: 1 µM H3K27me3 peptide was incubated with different concentrations of JMJD3 (1043- 1682) protein in 10 µl reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.02% Triton X-100, 1 mM TCEP, 50 µM 2-OG, 50 µM Ascorbate and 25 µM (NH4)2Fe(SO4)2·6H2O for 2 hours, then 10 micro;l H3K27me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

for Recombinant JMJD3 / KDM6B (1043-1682) proteinProtein Details

Recombinant JMJD3 / KMD6B (1043-1682) that includes amino acids 1043 - 1682 of human JMJD3 / KMD6B protein (accession number NP_001073893.1) was expressed in Baculovirus expression system and contains a C-terminal Flag tag with a molecular weight of 73.4 kDa. The recombinant protein is ≥ 90% pure by SDS-PAGE. 

 
JMJD3 / KDM6B (1043-1682) protein Coomassie gel.

Recombinant JMJD3 / KDM6B (1043-1682) SDS-PAGE gel. Recombinant JMJD3 was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.
MW: 73.4 kDa
Purity: > 90%

HTRF assay for JMJD3 / KDM6B (1043-1682) activity

HTRF assay for JMJD3 / KDM6B (1043-1682) activity.
1 µM histone H3K27me3 peptide was incubated with JMJD3 (1043-1682) protein in reaction buffer for 2 hours at room temperature. Reaction products were detected with H3K27me2 antibody.

HTRF assay for JMJD3 / KMD6B (1043-1682) activity

HTRF assay for JMJD3 / KDM6B (1043-1682) activity.
1 µM histone H3K27me3 peptide was incubated with JMJD3 (1043-1682) protein in reaction buffer for 2 hours at room temperature. Reaction products were detected with H3K27me2 antibody.

for Recombinant JMJD3 / KDM6B (1043-1682) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant JMJD3 / KDM6B (1043-1682) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant JMJD3 / KDM6B (1043-1682) protein
 

Data Thumbnails

JMJD3 / KDM6B (1043-1682) protein Coomassie gel.

JMJD3 /KDM6B (1043-1682) protein Coomassie stained gel.
(Click image to enlarge and see details).

HTRF assay for JMJD3 / KDM6B (1043-1682) activity

HTRF assay for JMJD3 / KDM6B (1043-1682) activity.
(Click image to enlarge and see details).

HTRF assay for JMJD3 / KMD6B (1043-1682) activity

HTRF assay for JMJD3 / KMD6B (1043-1682) activity. (Click image to enlarge)