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Recombinant OGT protein

Aliases: O-linked N-Acetylglucosamine Transferase, GlcNAc, EC 2.4.1.186 HINCUT-1, O-GLCNAC, HRNT1
Catalog No: 31524 Format: 20 µg $395 Buy Now
Catalog No: 31924 Format: 1 mg $3,500 Buy Now
Expressed In: Baculovirus Protein Species: Mouse

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for Recombinant OGT proteinContents

Recombinant full length Mouse OGT protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant OGT proteinBackground

OGT (O-linked N-acetylglucosamine (GlcNAc) transferase) catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to serine or threonine residues in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc). OGT can glycosylate a large and diverse number of proteins including HCF1, AKT1, MLL5 and histone H2B. It can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing. Specifically OGT has been shown to interact directly with TET2 and TET3, enzymes that catalyze the oxidation of 5-methylcytosine on DNA. The TET/OGT interaction leads to GlcNAcetylation of HCF1, a protein component of the COMPASS complex, which regulates H3K4 methylation and gene expression.

for Recombinant OGT proteinApplication Notes

Recombinant OGT protein is useful for the study of protein-protein interaction, enzyme kinetics, screening inhibitors, and selectivity profiling.

for Recombinant OGT proteinProtein Details

Recombinant mouse OGT was expressed in a baculovirus expression system as the full length protein (accession number NP_001277464.1) with an N-terminal FLAG-Tag. The molecular weight of the protein is 118.7 kDa. The purity of OGT protein is >90 by SDS-PAGE.

 
Recombinant OGT protein gel.

Recombinant OGT protein gel. OGT protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.

for Recombinant OGT proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant OGT proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant OGT protein
 

Data Thumbnails

Recombinant OGT protein gel.

OGT protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.
(Click image to enlarge and see details).