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Recombinant Proteins & Enzymes

Recombinant USP7 protein

Aliases: Ubiquitin Specific Peptidase 7, Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated)
Catalog No: 31525 Format: 20 µg $395 Buy Now
Catalog No: 31925 Format: 1 mg $3,500 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant USP7 proteinContents

Recombinant full length USP7 protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant USP7 proteinBackground

USP7 (Ubiquitin Specific Peptidase 7 (Herpes Virus-Associated)) belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. USP7 de-ubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, UHRF1, PTEN and DAXX. Together with DAXX, USP7 prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation. USP7 de-ubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis. USP7 also de-ubiquitinates FOXO4 and inhibits FOXO4-induced transcriptional activity. Together with DAXX, USP7 is involved in the de-ubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML. USP7 de-ubiquitinates UHRF1, which prevents its degradation and promoting DNA methylation by DNMT1.

for Recombinant USP7 proteinApplication Notes

This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

for Recombinant USP7 proteinProtein Details

Recombinant human USP7 was expressed in a baculovirus expression system as the full length protein (accession number NP_003461.2) with an N-terminal 6×His-Tag. The molecular weight of the protein is 131.3 kDa. The purity of USP7 protein is >80% by SDS-PAGE.

 
Recombinant USP7 protein gel.

Recombinant USP7 protein gel
8% SDS-PAGE gel stained with Coomassie Blue
MW: 131.3 kDa

Purity: >80%

DUB-Detector™ assay (Cat# 40110) for USP7 protein activity.

DUB-Detector assay (Cat. No. 40110) for USP7 protein activity
100 nM Fluorescent Substrate was incubated with different concentration of USP7 protein for 1 hour at room temperature. The fluorescent intensity of the reaction was measured with an excitation wavelength of 485 nm and an emission wavelength of 535nm.

DUB-Detector<sup>™</sup> assay (Cat# 40110) for USP7 protein activity.

DUB-Detector assay (Cat. No. 40110) for USP7 protein activity
100 nM Fluorescent Substrate was incubated with different concentration of USP7 protein for 1 hour at room temperature. The fluorescent intensity of the reaction was measured with an excitation wavelength of 485 nm and an emission wavelength of 535nm.

for Recombinant USP7 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant USP7 proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant USP7 protein
 

Data Thumbnails

Recombinant USP7 protein gel.

Recombinant USP7 protein gel.
(Click to enlarge and view details)

DUB-Detector™ assay (Cat# 40110) for USP7 protein activity.

DUB-Detectorassay (Cat# 40110) for USP7 protein activity.
(Click to enlarge and view details).

DUB-Detector<sup>™</sup> assay (Cat# 40110) for USP7 protein activity.

DUB-Detector assay (Cat# 40110) for USP7 protein activity.
(Click to enlarge and view details).