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Recombinant Proteins & Enzymes

Recombinant SIRT1 (193-741) protein

Aliases: Sirtuin 1, SIRT-1, SIR2-Like Protein, SIR2L1, SIR2alpha
Catalog No: 31533 Format: 100 µg $395 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant SIRT1 (193-741) proteinContents

Recombinant SIRT1 (193-741) protein is supplied in 25 mM Tris pH 8.0, 150 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant SIRT1 (193-741) proteinBackground

SIRT1 (Sirtuin 1, SIR2L1) is a member of the Sirtuin family of proteins related to the yeast Sir2 protein. Like Sir2 and other Sirtuins, SIRT1 is an NAD+-dependent histone and protein deacetylase. SIRT1 catalyzes the removal of acetyl groups from a variety of protein substrates (NBS1, p53, Ku70, etc.) as well as from Histone H4 Lys16 (H4K16). SIRT1 is involved in the regulation of a number of cellular processes, including transcription, metabolism, DNA repair and aging, and increased SIRT1 is associated with extended lifespan of C. elegans.

for Recombinant SIRT1 (193-741) proteinApplication Notes

Recombinant SIRT1 protein is useful for histone deacetylase assays and the study of DNA Repair.

for Recombinant SIRT1 (193-741) proteinProtein Details

Recombinant SIRT1 (193-741) protein that includes amino acids 193 - 741 of human SIRT1 protein (accession number NM_012238.4) was expressed in E. coli and contains an N-terminal GST-Tag with a molecular weight of 101.5 kDa. The purity of the protein is > 60% by SDS-PAGE.

 
SDS-PAGE Recombinant SIRT1 (193-741)

Recombinant SIRT1 (193-741)12.5% SDS-PAGE with Coomassie Blue staining.
MW: 101.5 kDa
Purity: >60%

HTRF assay for SIRT1 (193-741) activity

HTRF assay for SIRT1 (193-741) activity
3 µM histone peptide H3K9ac (1-21) was incubated with SIRT1 (193-741) in reaction buffer for 30 min at 37 °C. Anti-H3K9me0 antibody was used to detect reaction products.

HTRF assay for SIRT1 (193-741) activity

HTRF assay for SIRT1 (193-741) activity
3 µM histone peptide H3K9ac (1-21) was incubated with SIRT1 (193-741) in reaction buffer for 30 min at 37°C. Anti-H3K9me0 antibody was used to detect reaction products.

for Recombinant SIRT1 (193-741) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant SIRT1 (193-741) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant SIRT1 (193-741) protein
 

Data Thumbnails

SDS-PAGE Recombinant SIRT1 (193-741)

Recombinant SIRT1 Protein 12.5% SDS-PAGE with Coomassie Blue staining.
MW: 101.5 kDa
Purity: > 60%
(Click image to enlarge and see details).

HTRF assay for SIRT1 (193-741) activity

3 µM histone peptide H3K9ac (1-21) was incubated with SIRT1 (193-741) in reaction buffer for 30 min at 37 °C. Anti-H3K9me0 antibody was used to detect reaction products.
(Click image to enlarge and see details).

HTRF assay for SIRT1 (193-741) activity

3 µM histone peptide H3K9ac (1-21) was incubated with SIRT1 (193-741) in reaction buffer for 30 min at 37°C. Anti-H3K9me0 antibody was used to detect reaction products.
(Click image to enlarge and see details).