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Recombinant Proteins & Enzymes

Recombinant HDAC9 (604-1066) protein

Aliases: Histone Deacetylase 9, HDAC-9, Histone Deacetylase 7B, HDAC7B, HD9
Catalog No: 31537 Format: 20 µg $395 Buy Now
Catalog No: 31937 Format: 1 mg $3,500 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant HDAC9 (604-1066) proteinContents

Recombinant HDAC9 (604-1066) protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant HDAC9 (604-1066) proteinBackground

HDAC9 (Histone Deacetylase 9) is a member of the class IIa mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: HDAC class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, 9 and 10), class III (SIRT1-7) and class IV (HDAC11).

In humans, HDAC9 is expressed in a wide variety of tissues. This gene is orthologous to the Xenopus and mouse MITR genes. HDAC9 represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDAC1 and/or HDAC3. HDAC9 may play a role in hematopoiesis. It seems to inhibit skeletal myogenesis and to be involved in heart development. HDAC9 protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.

for Recombinant HDAC9 (604-1066) proteinApplication Notes

Recombinant HDAC9 (604-1066) protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiing.

for Recombinant HDAC9 (604-1066) proteinProtein Details

Recombinant HDAC9 (604-1066) protein that includes amino acids 604-1066 of human HDAC9 protein (accession number NP_848510.1) was expressed in Sf9 cells and contains an C-terminal FLAG-Tag with a molecular weight of 60.3 kDa. The purity of HDAC9 protein is >80% by SDS-PAGE.

 
Recombinant HDAC9 (604-1066) protein gel.

Recombinant HDAC9 (604-1066) protein gel.
HDAC9 (604-1066) protein was run on an 8% SDS- PAGE gel and stained with Coomassie Blue.
MW: 60.3 kDa
Purity: > 80%

HDAC-Glo™ Class IIa Assay for HDAC9 activity

HDAC-Glo™ Class IIa Assay for HDAC9 activity.
Assay was performed using HDAC-Glo Class IIa Assay from Promega. 3.5 µM substrate was incubated with HDAC9 proteins and 1/20,000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.

HDAC-Glo™ Class IIa Assay for HDAC9 activity

HDAC-Glo™ Class IIa Assay for HDAC9 activity.
Assay was performed using HDAC-Glo Class IIa Assay from Promega. 3.5 µM substrate was incubated with HDAC9 proteins and 1/20,000 developer reagent at room temperature, then luminescence was detected after incubation for 20 min.

for Recombinant HDAC9 (604-1066) proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant HDAC9 (604-1066) proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant HDAC9 (604-1066) protein
 

Data Thumbnails

Recombinant HDAC9 (604-1066) protein gel.

Recombinant HDAC9 (604-1066) protein gel.
(Click to enlarge and view details).

HDAC-Glo™ Class IIa Assay for HDAC9 activity

HDAC-Glo™ Class IIa Assay for HDAC9 activity.
(Click to enlarge and view details).

HDAC-Glo™ Class IIa Assay for HDAC9 activity

HDAC-Glo™ Class IIa Assay for HDAC9 activity.
(Click to enlarge and view details).