Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Recombinant Proteins & Enzymes

Recombinant NONO protein

Aliases: Non-POU Domain Containing, Octamer-Binding, NMT55, P54NRB , Nuclear RNA-Binding Protein,
Catalog No: 31539 Format: 20 µg $395 Buy Now
Expressed In: Baculovirus Protein Species: Human

Request a quote for a bulk orderBulk Order Button


for Recombinant NONO proteinContents

Recombinant full length NONO protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant NONO proteinBackground

NONO (non-POU domain containing, octamer-binding, p54(nrb), NRB54, NMT55) is a DNA- and RNA binding protein and involved in several nuclear processes. NONO binds the conventional octamer sequence in double-stranded DNA. It also binds single-stranded DNA and RNA at a site independent of the duplex site. This gene also encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma.

for Recombinant NONO proteinApplication Notes

Recombinant NONO is suitable for use in RNA binding, assays and protein-protein interaction.

for Recombinant NONO proteinProtein Details

Recombinant human NONO was expressed in a baculovirus expression system as the full length protein (accession number NP_031389.3) with an N-terminal FLAG-Tag. The molecular weight of the protein is 57.2 kDa. The NONO protein is >90% pure by SDS-PAGE.

 
Recombinant NONO protein gel.

Recombinant NONO protein gel.NONO protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.

for Recombinant NONO proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant NONO proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant NONO protein
 

Data Thumbnails

Recombinant NONO protein gel.

NONO protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.
(Click image to enlarge and see details).