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Recombinant Proteins & Enzymes

Recombinant Histone H3.3 (K27M)

Aliases: Histone H3, Histone H-3
Catalog No: 31552 Format: 100 µg $95 Buy Now
Catalog No: 31952 Format: 1 mg $500 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant Histone H3.3 (K27M)Contents

Recombinant Histone H3.3 (K27M) is supplied in 50 mM Tris pH 8.0, 150 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant Histone H3.3 (K27M)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.

Histone H3.1 and Histone H3.3 are the two main Histone H3 variants found in plants and animals. They are known to be important for gene regulation. Histone H3.1 and H3.3 have been shown to demonstrate unique genomic localization patterns thought to be associated with their specific functions in regulation of gene activity. Specifically, Histone H3.1 localization is found to coincide with genomic regions containing chromatin repressive marks (H3K9me3, H3K27me3 and DNA methylation), whereas Histone H3.3 primarily colocalizes with marks associated with gene activation (H3K4me3, H2BK120ub1, and RNA pol II occupancy). Deposition of the Histone H3.1 variant into the nucleosome correlates with the canonical DNA synthesis-dependent deposition pathway, whereas Histone H3.3 primarily serves as the replacement Histone H3 variant outside of S-phase, such as during gene transcription.

Histone H3.3 point mutations (K27 and G34) are present in 1/3 of pediatric glioblastomas. Up to 78% of diffuse intrinsic pontine gliomas (DIPGs) carries K27M and 36% of non-brainstem gliomas carries either K27M or G34R/V mutations. H3.3K27M expression synergizes with p53 loss and PDGFRA activation in neural progenitor cells derived from human embryonic stem cells, resulting in neoplastic transformation.

for Recombinant Histone H3.3 (K27M)Application Notes

Recombinant Histone H3.3 (K27M) is suitable for use as substrate for histone modification enzymes, or to generate chromatin in vitro.

for Recombinant Histone H3.3 (K27M)Protein Details

Recombinant Histone H3.3 (K27M) was expressed in E. coli cells as full length protein (assession number: NP_002098.1) with a point mutant Lys27Met and has an observed molecular weight of 15.4 kDa. The recombinant histone H3.3 (K27M) is >85% pure by SDS-PAGE.

 
Recombinant Histone H3.3 (K36M) gel.

Recombinant Histone H3.3 (K36M) gel.
Histone H3.3 (K36M) was run on an 12% SDS-PAGE gel and stained with Coomassie Blue. The purity of Histone H3.3 (K36M): ≥ 85%

for Recombinant Histone H3.3 (K27M)Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Histone H3.3 (K27M)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Recombinant Histone H3.3 (K27M)
 

Data Thumbnails

Recombinant Histone H3.3 (K36M) gel.

Histone H3.3 (K27M) was run on an 12% SDS-PAGE gel and stained with Coomassie Blue. The purity of Histone H3.3 (K27M): ≥ 85%
(Click image to enlarge and see details).