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Recombinant Proteins & Enzymes

Recombinant METTL3 protein

Catalog No: 31567 Format: 20 µg $395 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant METTL3 proteinContents

Recombinant METTL3 protein is supplied at a concentration of 0.35 µg/µl in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP.

for Recombinant METTL3 proteinBackground

N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also occurs in other RNA molecules, such as primary miRNA (pri-miRNAs).
METTL3 (methyltransferase-like 3, also known as IME4, M6A, MT-A70) forms a stable N6-methyltransferase heterodimer complex with METTL14, which catalyzes the generation of m6A modification on mammalian nuclear RNAs. METTL3 is the catalytically active subunit, while METTL14 plays a structural role critical for substrate recognition. METTL3 promotes translation by interacting with the translation initiation machinery in the cytoplasm. Its overexpression in a number of cancer cells suggests that it may participate to cancer cell proliferation by promoting mRNA translation.

for Recombinant METTL3 proteinApplication Notes

Recombinant METTL3 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
Activity Assay Conditions: 1 µM substrate ssRNA oligos (5’-GUUGCCUGUUCGUGUUGGACUUGCCUGU-3’) and 1 µM SAM were incubated with METTL3 protein in EPI diluent buffer provided by Cisbio at room temperature for 1 hour (0.2 U/µL RNase inhibitor was added in this system). SAH-d2 and anti-SAH-K were added to the products, then HTRF signals were read after another 1 hour incubation. EPIgeneous™ Methyltransferase Assay kit from Cisbio was used for activity assay.

for Recombinant METTL3 proteinProtein Details

Recombinant METTL3 protein was expressed in a Baculovirus expression system as the full length protein (accession number NP_062826.2) with an N-terminal FLAG-Tag. The molecular weight of METTL3 is 65.5 kDa.

 
Recombinant METTL3 protein gel.

Recombinant METTL3 protein gel.
Recombinant METTL3 protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.
MW: 65.5 kDa
Purity: > 95%

Recombinant METTL3 protein HTRF assay.

Recombinant METTL3 protein HTRF assay
1 µM substrate ssRNA oligos and 1 µM SAM were incubated with METTL3 protein in EPI buffer at RT for 1 hour. SAH-d2 and anti-SAH-K were added to the products, HTRF signals read after 1 hr. Cisbio EPIgeneous™ Methyltransferase Assay kit was used.

for Recombinant METTL3 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant METTL3 proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant METTL3 protein
 

Data Thumbnails

Recombinant METTL3 protein gel.

Recombinant METTL3 protein gel.
(Click to enlarge and view details).

Recombinant METTL3 protein HTRF assay.

Recombinant METTL3 protein HTRF assay
(Click to enlarge and view details).