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Recombinant Proteins & Enzymes

Recombinant METTL14 protein

Catalog No: 31568 Format: 20 µg $395 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant METTL14 proteinContents

Recombinant METTL14 protein is supplied at a concentration of 0.2 µg/µl in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP.

for Recombinant METTL14 proteinBackground

N6-methylated adenine (m6A) is prevalently present in nearly all RNA types and can be found in all organisms from bacteria to humans. It preferentially appears around stop codons and within long internal exons in mammalian messager RNAs. m6A plays an important role in the efficiency of mRNA splicing, processing, translation efficiency, editing and mRNA stability. m6A also takes place in other RNA molecules, such as primary miRNA (pri-miRNAs).
METTL14 (methyltransferase-like 14) forms a stable N6-methyltransferase heterodimer complex with METTL3, which catalyzes the generation of m6A modification on mammalian nuclear RNAs. METTL3 is the catalytically active subunit, while METTL14 plays a structural role critical for substrate recognition.

for Recombinant METTL14 proteinApplication Notes

Recombinant METTL14 protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.

for Recombinant METTL14 proteinProtein Details

Recombinant METTL14 protein was expressed in a Baculovirus expression system as the full length protein (accession number NP_066012.1) with an N-terminal FLAG-Tag. The molecular weight of METTL14 is 53.3 kDa.

 
Recombinant METTL14 protein gel.

Recombinant METTL14 protein gel
Recombinant METTL14 was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.
MW: 53.3 kDa
Purity: > 75%

for Recombinant METTL14 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant METTL14 proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant METTL14 protein
 

Data Thumbnails

Recombinant METTL14 protein gel.

Recombinant METTL14 protein gel.
(Click to enlarge and view details).