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Recombinant FTO protein

Catalog No: 31572 Format: 20 µg $395 Buy Now
Catalog No: 31972 Format: 1 mg $3,500 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant FTO proteinContents

Recombinant FTO protein is supplied at a concentration of 0.25 µg/µl in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP.

for Recombinant FTO proteinBackground

FTO (Alpha-Ketoglutarate Dependent Dioxygenase, also known as GDFD, ALKBH9, BMIQ14) is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily. FTO specifically demethylates m6A RNA. FTO plays an important role in the regulation of the global metabolic rate, energy expenditure and energy homeostasis. It is involved in the regulation of thermogenesis and the control of adipocyte differentiation into brown or white fat cells.

for Recombinant FTO proteinApplication Notes

Recombinant FTO protein is suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling.
3 µM m6A ssDNA oligos (DNA sequence: 5’-GTTGCCTGTTCGTGTTGG/m6A/ CTTGCCTGT-3’) were incubated with FTO protein in reaction buffer including 50 mM HEPES pH 7.5, 100 µM 2OG, 100 µM Ascorbate, 50 µM (NH4)2Fe(SO4)2·6H2O and 1 mM TCEP for 2 hours at room temperature. Dot-Blot was used for activity detection.

for Recombinant FTO proteinProtein Details

Recombinant FTO protein was expressed in a Baculovirus expression system as the full length protein (accession number XP_011521615.1) with an N-terminal FLAG-Tag. The molecular weight of FTO is 60.4 kDa.

 
Recombinant FTO protein, SDS-PAGE gel.

Recombinant FTO protein, SDS-PAGE gel.
Recombinant FTO protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.
MW: 60.4 kDa
Purity: > 95%

Dot-Blot assay for Recombinant FTO protein activity.

Recombinant FTO protein activity.
3 µM m6A ssDNA oligos (sequence: 5’-GTTGCCTGTTCGTGTTGG/m6A/CTTGCCTGT-3’) were incubated with FTO protein in reaction buffer for 2 hr. at RT. Reaction products spotted onto nylon membrane were detected with Anti-m6A antibody (#31495). FTO alone was used as negative control.

for Recombinant FTO proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant FTO proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant FTO protein
 

Data Thumbnails

Recombinant FTO protein, SDS-PAGE gel.

Recombinant FTO protein, SDS-PAGE gel.
(Click to enlarge and view details)

Dot-Blot assay for Recombinant FTO protein activity.

Dot-Blot assay for Recombinant FTO protein activity.
(Click to enlarge and view details).