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Recombinant Proteins & Enzymes

Recombinant Histone H3K36me3 (MLA)

Aliases: H3K36me3, Histone H3, methylated lysine analog
Catalog No: 31580 Format: 100 µg $250 Buy Now
Expressed In: E. coli Protein Species: Human

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Methylated Lysine Analog

Recombinant Histone H3K36me3 (MLA) has been generated using the patented Methylated Lysine Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine ϒ-methylene. The MLA technique provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112.

for Recombinant Histone H3K36me3 (MLA)Contents

Recombinant Histone H3K36me3 (MLA) is supplied in 50 mM Tris-HCl, pH 8.0, 150 mM NaCl and 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant Histone H3K36me3 (MLA)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.

Recombinant Histone H3K36me3 (MLA) has been generated using the patented Methylated Lysine Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine Υ-methylene. The MLA technique provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112.

for Recombinant Histone H3K36me3 (MLA)Application Notes

Recombinant histones are suitable for use as positive controls in the analysis of histone post- translational modi cations, as substrates for histone modifiation enzymes, or to generate chromatin in vitro.

for Recombinant Histone H3K36me3 (MLA)Protein Details

Recombinant human Histone H3 trimethyl Lys36 (H3K36me3) (accession number NP_003520.1) was expressed in E. coli cells and contains two substitutions (cysteine to serine at amino acid 96 and cysteine to alanine at amino acid 110). Recombinant methylated histones are specifically methylated via a chemical alkylation reaction that introduces a methyl lysine analog (MLA). The molecular weight of the recombinant histone is 15,285 Daltons. The recombinant histone is >92% pure by SDS-PAGE.

 
Recombinant Histone H3K36me3 (MLA) gel.

Recombinant Histone H3K36me3 (MLA) gel. Histone H3K36me3 (MLA) was run on a 12.5% SDS- PAGE gel and stained with Coomassie Blue.

Mass Spec analysis for Recombinant Histone H3K36me3 (MLA) gel.

Mass Spec analysis for Recombinant Histone H3K36me3 (MLA) gel.
Mass Spec analysis shows that almost all of histones. are K36me3 histones.

for Recombinant Histone H3K36me3 (MLA)Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Histone H3K36me3 (MLA)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant Histone H3K36me3 (MLA)
 

Data Thumbnails

Recombinant Histone H3K36me3 (MLA) gel.

Recombinant Histone H3K36me3 (MLA) gel.
(Click to enlarge and view details).

Mass Spec analysis for Recombinant Histone H3K36me3 (MLA) gel.

ESI-TOF Mass Spec analysis of Recombinant Histone H3K36me3 (MLA)
Expected mass: 15285 Da
Observed mass: 15285 Da