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Recombinant Proteins & Enzymes

Recombinant TDO2 protein

Catalog No: 31588 Format: 50 µg $395 Buy Now
Catalog No: 31988 Format: 1 mg $2,600 Buy Now
Expressed In: E. coli Protein Species: Human

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for Recombinant TDO2 proteinContents

Recombinant TDO2 protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, 0.5 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant TDO2 proteinBackground

TDO2 (Tryptophan 2,3-Dioxygenase, also known as TRPO, TDO, TO, TPH2) is a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. TDO2 has a broad specificity towards tryptamine and derivatives including D- and L-tryptophan, 5-hydroxytryptophan and serotonin. The excessive activation of TDO2 may also play a role in cancer through the suppression of antitumor immune responses.

for Recombinant TDO2 proteinApplication Notes

This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Activity Assay Conditions: 400 µM L-tryptophan was incubated with different concentrations of TDO2 protein in reaction buffer including 50 mM potassium phosphate pH 6.5, 20 mM ascorbic acid, 10 µM methylene blue and 200 µg/ml catalase for 1 hr at 37°C. The total 200 µl reaction was stopped by the addition of 40 µl 30% (w/v) trichloroacetic acid and heated at 65°C for 15 min. After cooled down to 4°C, the reaction was centrifuged at 1,125 g for 10 min. 100 µl supernatant was transferred into 96-well plate and mixed with 100 µl of 2% (w/v) p-DMAB in acetic acid. The yellow pigment derived from kynurenine was measured at 490 nm. 

for Recombinant TDO2 proteinProtein Details

Recombinant TDO2 was expressed in E. coli cells as the full length protein (accession number NP_005642.1) with an N-terminal 6×His tag. The molecular weight of the protein is 51.4 kDa.

 
Recombinant TDO2 protein gel

Recombinant TDO2 protein gel
12.5% SDS-PAGE Coomassie staining
MW: 51.4 kDa
Purity: > 85%

Recombinant TDO2 protein activity assay

Recombinant TDO2 protein activity assay
L-tryptophan was incubated with different concentrations of TDO2 protein in reaction buffer for 1 hr at 37°C. The reaction was stopped by addition of trichloroacetic acid and heated at 65°C for 15 min. After cooling, the reaction was centrifuged and supernatant was transferred into 96-well plate and mixed with p-DMAB in acetic acid. The yellow pigment derived from kynurenine was measured at 490 nm. 

Recombinant TDO2 protein activity assay

Recombinant TDO2 protein activity assay
L-tryptophan was incubated with different concentrations of TDO2 protein in reaction buffer for 1 hr at 37°C. The reaction was stopped by addition of trichloroacetic acid and heated at 65°C for 15 min. After cooling, the reaction was centrifuged and supernatant was transferred into 96-well plate and mixed with p-DMAB in acetic acid. The yellow pigment derived from kynurenine was measured at 490 nm. 

for Recombinant TDO2 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant TDO2 proteinGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant TDO2 protein
 

Data Thumbnails

Recombinant TDO2 protein gel

Recombinant TDO2 protein gel
(Click to enlarge and view details)

Recombinant TDO2 protein activity assay

Recombinant TDO2 protein activity assay
(Click to enlarge and view details)

Recombinant TDO2 protein activity assay

Recombinant TDO2 protein activity assay
(Click to enlarge and view details)