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Recombinant Proteins & Enzymes

Recombinant HDAC3 / NCOR2 complex, His-Tag

Catalog No: 31609 Format: 50 µg $395 Buy Now
Catalog No: 31709 Format: 1 mg $3,950 Buy Now
Expressed In: Baculovirus Protein Species: Human

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for Recombinant HDAC3 / NCOR2 complex, His-TagContents

Recombinant HDAC3 / NCOR2 Complex, His-tag is supplied in 50 mM Tris-HCl pH 8.0, 300mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

for Recombinant HDAC3 / NCOR2 complex, His-TagBackground

HDAC3 (Histone Deacetylase 3, also designated HD3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

In vivo, acetylation is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). The HDACs are grouped into four classes, on the basis of similarity to yeast counterparts: class I (HDAC1, HDAC2, HDAC3 and HDAC8), class II (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9 and 10), class III (SIRT1-7) and class IV (HDAC11).

By forming multi-protein complexes with the co-repressors NCOR2, HDAC3 regulates the transcription of a plethora of genes. A growing list of non-histone proteins extends the role of HDAC3 beyond transcriptional repression. HDAC1, HDAC2 and HDAC3 are also ubiquitously expressed and can deacetylate both H3 and H4 in free histones or nucleosome substrate. 

for Recombinant HDAC3 / NCOR2 complex, His-TagApplication Notes

This protein is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

HDAC Activity Assay Conditions: 3 µM H3K9ac peptide was incubated with different concentrations of HDAC3 / NCOR2 Complex, His-tag in reaction buffer including 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA for 30 minutes at 37°C. HTRF assay was used for activity detection.

Active Motif has a collection of small-molecule epigenetic modulators that target HDACs - click here to view the list.

for Recombinant HDAC3 / NCOR2 complex, His-TagProtein Details

Recombinant HDAC3 / NCOR2 Complex, His-tag that includes full length human HDAC3 protein (accession number NP_003874.2) with a C-terminal 6×His tag and human NCOR2 amino acids 395-489 (accession number NP_006303.4) with an N-terminal GST tag was expressed in Sf9 cells. The molecular weights of HDAC3 and NCOR2 (395-489) are 49.7 kDa and 37.9 kDa, respectively.

 
Recombinant HDAC3 / NCOR2 complex, His-tag

Recombinant HDAC3 / NCOR2 complex, His-tag
12% SDS-PAGE Coomassie staining
MW: HDAC3: 49.7 kDa
MW: NCOR2: 37.9 kDa
Purity: > 85% 

HTRF assay for HDAC3 / NCOR2 Complex, His-tag activity

HTRF assay for HDAC3 / NCOR2 Complex, His-tag activity
3 µM histone H3K9ac peptide was incubated with different concentrations of HDAC3 / NCOR2 Complex, His-tag in reaction buffer for 30 minutes at 37°C. H3K9me0-Eu antibody was used to detect the reaction products.

HTRF assay for HDAC3 / NCOR2 Complex, His-tag activity

HTRF assay for HDAC3 / NCOR2 Complex, His-tag activity
3 µM histone H3K9ac peptide was incubated with different concentrations of HDAC3 / NCOR2 Complex, His-tag in reaction buffer for 30 minutes at 37°C. H3K9me0-Eu antibody was used to detect the reaction products.

for Recombinant HDAC3 / NCOR2 complex, His-TagStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant HDAC3 / NCOR2 complex, His-TagGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant HDAC3 / NCOR2 complex, His-Tag
 

Data Thumbnails

Recombinant HDAC3 / NCOR2 complex, His-tag

Recombinant HDAC3 / NCOR2 complex, His-tag
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HTRF assay for HDAC3 / NCOR2 Complex, His-tag activity

HTRF assay for HDAC3 / NCOR2 Complex, His-tag activity
(Click to enlarge and view details)

HTRF assay for HDAC3 / NCOR2 Complex, His-tag activity

HTRF assay for HDAC3 / NCOR2 Complex, His-tag activity
(Click to enlarge and view details)