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Recombinant Proteins & Enzymes

Recombinant Histone H3K79me1 (MLA)

Catalog No: 31623 Format: 100 µg $250 Buy Now
Catalog No: 31723 Format: 1 mg $850 Buy Now
Expressed In: E. coli Protein Species: Human

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Methylated Lysine Analog

Recombinant Histone H3K79me1 (MLA) has been generated using the patented Methylated Lysine Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine ϒ-methylene. The MLA technique provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112.

for Recombinant Histone H3K79me1 (MLA)Contents

Recombinant Histone H3K79me1 (MLA) is supplied in 50 mM Tris-HCl, pH 8.0, 150 mM NaCl and 5% glycerol.

for Recombinant Histone H3K79me1 (MLA)Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.

Recombinant Histone H3K79me1 (MLA) has been generated using the patented Methylated Lysine Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine ϒ-methylene. The MLA technique provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112. 

for Recombinant Histone H3K79me1 (MLA)Application Notes

Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.

for Recombinant Histone H3K79me1 (MLA)Protein Details

Recombinant human Histone H3 monomethyl Lys79 (H3K79me1) (accession number NP_003520.1) was expressed in E. coli cells and contains two substitutions (cysteine to serine at amino acid 96 and cysteine to alanine at amino acid 110). Recombinant methylated histones are specifically methylated via a chemical alkylation reaction that introduces a methyl lysine analog (MLA). The molecular weight of the protein is 15257 Daltons.

 
Recombinant Histone H3K79me1 (MLA) protein gel.

Recombinant Histone H3K79me1 (MLA) protein gel.
12% SDS-PAGE Coomassie staining.
Purity: > 80%

ESI-TOF Mass Spec analysis for Recombinant Histone H3K79me1 (MLA).

ESI-TOF Mass Spec analysis for Recombinant Histone H3K79me1 (MLA).
Expected mass: 15257 Da
Observed mass: 15257 Da

for Recombinant Histone H3K79me1 (MLA)Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Histone H3K79me1 (MLA)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant Histone H3K79me1 (MLA)
 

Data Thumbnails

Recombinant Histone H3K79me1 (MLA) protein gel.

Recombinant Histone H3K79me1 (MLA) protein gel.
(Click to enlarge and view details)

ESI-TOF Mass Spec analysis for Recombinant Histone H3K79me1 (MLA).

ESI-TOF Mass Spec analysis for Recombinant Histone H3K79me1 (MLA).
(Click to enlarge and view details)