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Recombinant Proteins & Enzymes

Recombinant Histone H4K20me3 (MLA), His-Tag

Catalog No: 31626 Format: 100 µg $250 Buy Now
Catalog No: 31726 Format: 1 mg $850 Buy Now
Expressed In: E. coli Protein Species: Human

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Methylated Lysine Analog

Recombinant Histone H4K20me3 (MLA), His-Tag has been generated using the patented Methylated Lysine Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and chemically similar to lysine, though it contains a sulfide substitution in place of the lysine ϒ-methylene. The MLA technique provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112.

for Recombinant Histone H4K20me3 (MLA), His-TagContents

Recombinant Histone H4K20me3 (MLA), His-Tag is supplied in 25 mM Tris-HCl pH 7.4, 150 mM NaCl and 10% glycerol.

for Recombinant Histone H4K20me3 (MLA), His-TagBackground

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/ exit points.

Recombinant Histone H4K20me3 (MLA) has been generated using the patented Methylated Lysine Analog (MLA) technology. In MLA, methylated histones are generated via a chemical alkylation reaction that substitutes a methylated analog of lysine, aminoethylcysteine, for the existing lysine at the desired residue. Aminoethylcysteine is structurally and cϒ-provides precise control over the site and degree of methylation. The MLA technology is covered under U.S. Patent No. 8,278,112.

for Recombinant Histone H4K20me3 (MLA), His-TagApplication Notes

Recombinant Histone H4K20me3 (MLA), His-Tag is suitable for use as substrate for histone modification enzymes, or to generate chromatin in vitro.

for Recombinant Histone H4K20me3 (MLA), His-TagProtein Details

Recombinant Histone H4K20me3 (MLA), His-Tag was expressed in E. coli cells as full length protein (assession number: NP_003539.1) with a C-terminal 6×His tag, of which the Lysine 20 was mutated to Cysteine and then tri-methylated via MLA technology. The molecular weight of this protein is 12362 Da.

 
Recombinant Histone H4K20me3 (MLA), His-Tag protein gel

Recombinant Histone H4K20me3 (MLA), His-Tag protein gel
13% SDS-PAGE gel, stained with Coomassie Blue.
MW: 12.4 kDa
Purity: > 90%

ESI-TOF Mass Spec analysis for Recombinant Histone H4K20me3 (MLA), His-Tag

ESI-TOF Mass Spec analysis for Recombinant Histone H4K20me3 (MLA), His-Tag

Expected mass: 12362 Da
Observed mass: 12362 Da

for Recombinant Histone H4K20me3 (MLA), His-TagStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant Histone H4K20me3 (MLA), His-TagGuarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

 

Technical Data Sheet

Recombinant Histone H4K20me3 (MLA), His-Tag
 

Data Thumbnails

Recombinant Histone H4K20me3 (MLA), His-Tag protein gel

Recombinant Histone H4K20me3 (MLA), His-Tag protein gel
(Click to enlarge and view details)

ESI-TOF Mass Spec analysis for Recombinant Histone H4K20me3 (MLA), His-Tag

ESI-TOF Mass Spec analysis for Recombinant Histone H4K20me3 (MLA), His-Tag
(Click to enlarge and view details)