F9 nuclear extract

for F9 nuclear extractContents

200 µg of F9 nuclear extract at 2.5 µg/µl.

for F9 nuclear extractBackground

F9 nuclear extract was prepared from cell cultures of the mouse teratocarcinoma F9 stem cell line. This cell line expresses characteristics of early mouse embryonal cells and is considered to be nullipotent because of its inability to spontaneously differentiate. However, introduction of chemical agents, such as retinoic acid (RA), or transfection with oncogenic sequences, such as SV40 large T antigen, can induce differentiation of these cells. F9 cells readily differentiate into an endodermal-like derivative phenotype, but possess the ability to differentiate into derivatives of all three germ layers under the appropriate culture conditions, suggesting that these cells may be pluripotent. The F9 stem cell line is widely used in research to study the biomolecular mechanisms underlying differentiation.

for F9 nuclear extractApplication Notes

F9 nuclear extract is specifically recommended for studies related to differentiation and in stem cell research.

for F9 nuclear extractExtract Origin

Murine testes (Embryonal Carcinoma)

for F9 nuclear extractExtract Composition

F9 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl₂, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

for F9 nuclear extractQuality Control

Each lot has been tested for GATA-4 activation by using TransAM^® Kits.

for F9 nuclear extractStorage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for F9 nuclear extractGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.