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Cell, Acid & Tissue Extracts

Hep G2 nuclear extract

Catalog No: 36011 Format: 200 µg $165 Buy Now

for Hep G2 nuclear extractContents

2 x 100 µg of Hep G2 nuclear extract at 2.5 µg/µl.

for Hep G2 nuclear extractBackground

Hep G2 nuclear extract was prepared from cell cultures of the human hepatocellular liver carcinoma Hep G2 cell line. These cells are epithelial in morphology and can be induced to form polarized epithelium under appropriate culture conditions. Hep G2 cells are most commonly used for studies related to hepatocyte function. Because of their ability to differentiate into polarized epithelium, these cells are often used to investigate intracellular protein trafficking, specifically in relation to human liver diseases. Hep G2 is the most commonly used cell line for studying the regulation of hepatic protein synthesis, particularly the synthesis of acute phase proteins after the onset of a systemic inflammatory response. In addition, these cells are used to study liver metabolism, liver cancers, liver regeneration, liver cytotoxicity, apoptosis and as a model for hepatitis B virus (HBV) viral etiology.

for Hep G2 nuclear extractApplication Notes

Hep G2 nuclear extract is specifically recommended for studies related to 1) human liver diseases, cancers and drug therapies, 2) HBV etiology, 3) signaling pathways, and 4) apoptosis.

for Hep G2 nuclear extractExtract Origin

Human Liver (hepatocellular carcinoma)

for Hep G2 nuclear extractExtract Composition

Hep G2 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

for Hep G2 nuclear extractQuality Control

Extracts have been quality control tested by Western blot and TransAM® HNF Family Kits.

 
Monitoring HNF-1 activity with the TransAM HNF-1 Kit.

Figure 1: Monitoring HNF-1 activity with the TransAM HNF-1 Kit.
Different amounts of nuclear extracts from untreated Hep G2 and HeLa cells are tested for HNF-1 activity using the TransAM HNF-1 Kit.

for Hep G2 nuclear extractStorage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for Hep G2 nuclear extractGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Hep G2 nuclear extract
 

Data Thumbnails

Monitoring HNF-1 activity with the TransAM HNF-1 Kit.

Assay of HNF-1 activation using the TransAM FHNF-1 Kit.
(Click image to enlarge and see details.)