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Jurkat nuclear extract (TPA + CI stimulated)

Catalog No: 36013

Format: 200 µg

Contents:

2 x 100 µg of Jurkat nuclear extract (TPA + CI stimulated) at 2.5 µg/µl.

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$ 110

Extract Origin:

Human T lymphoblast; acute T cell leukemia

Extract Composition:

Jurkat nuclear extract (TPA + CI stimulated) is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl₂, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). Cells were cultured in medium supplemented with 50 ng/ml TPA (phorbol, 12-myristate, 13 acetate) and 0.5 µM calcium ionophore A23187 (CI) for two hours at 37°C immediately prior to harvesting.

Quality Control:

Extracts have been quality control tested by Western blot and TransAM™ Transcription Factor ELISAs.

Storage:

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

Guarantee:

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

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