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Cell, Acid & Tissue Extracts

MCF-7 nuclear extract

Catalog No: 36017 Format: 200 µg $165 Buy Now

for MCF-7 nuclear extractContents

2 x 100 µg of MCF-7 nuclear extract at 2.5 µg/µl.

for MCF-7 nuclear extractBackground

MCF-7 nuclear extract was prepared from cell cultures of the human MCF-7 breast adenocarcinoma cancer cell line. The MCF-7 cell line was originally derived from a pleural effusion of a 69-year old Caucasian woman with metastatic breast cancer. MCF-7 cells are the best characterized and most commonly utilized cell lines in in vitro breast cancer studies. The MCF-7 cell line has retained several characteristics that are particular to mammary epithelium, including the ability to grow as a monolayer, form domes, and respond to hormones. These cells also display a low metastatic potential, leading to the assumption that they represent an early epithelial adenocarcinoma phenotype. These characteristics make MCF-7 cells an ideal model system to study malignant progression in relation to breast cancer.

for MCF-7 nuclear extractApplication Notes

MCF-7 nuclear extract is specifically recommended for studies related to breast cancer research.

for MCF-7 nuclear extractExtract Origin

Human breast carcinoma

for MCF-7 nuclear extractExtract Composition

MCF-7 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

for MCF-7 nuclear extractQuality Control

Extracts have been quality control tested by Western blot and TransAM® Transcription Factor ELISAs.

 
Figure 1: Monitoring ERα activation with the TransAM ER Kit.

Figure 1: Monitoring ERα activation with the TransAM ER Kit.
Different amounts of untreated and H2O2 post-treated nuclear extracts from MCF-7 cells are tested for ER activity using the TransAM ER Kit.

Figure 1: Monitoring Sp1 and Sp3 activity with the TransAM Sp1/Sp3 Kit.

Figure 1: Monitoring Sp1 and Sp3 activity with the TransAM Sp1/Sp3 Kit.
Different amounts of untreated and H2O2-post treated nuclear extracts from MCF-7 cells are tested for Sp1 and Sp3 activity using the TransAM Sp1/Sp3 Kit.

for MCF-7 nuclear extractStorage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for MCF-7 nuclear extractGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

MCF-7 nuclear extract
 

Data Thumbnails

Figure 1: Monitoring ERα activation with the TransAM ER Kit.

Assay of ERα activation using the TransAM ER Kit.
(Click image to enlarge and see details.)

Figure 1: Monitoring Sp1 and Sp3 activity with the TransAM Sp1/Sp3 Kit.

Assay of Sp1 and Sp3 activation using the TransAM Sp1/Sp3 Kit.
(Click image to enlarge and see details.)