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Cell, Acid & Tissue Extracts

PC-12 nuclear extract

Catalog No: 36022 Format: 200 µg $165 Buy Now

for PC-12 nuclear extractContents

2 x 100 µg of PC-12 nuclear extract at 2.5 µg/µl.

for PC-12 nuclear extractBackground

PC-12 nuclear extract was prepared from cell cultures of the rat adrenal pheochromocytoma PC-12 cell line. This cell line originated from a catecholamine-secreting tumor (pheochromocytoma) of the rat adrenal medulla. In addition to catecholamine, PC-12 cells also produce neurotransmitters. PC-12 cells are frequently used as a model system for neuronal differentiation because of their ability to differentiate into a sympathetic neuronal phenotype in response to nerve growth factor, fibroblast growth factor and, under certain conditions, epidermal growth factor.

for PC-12 nuclear extractApplication Notes

PC-12 nuclear extract is specifically recommended for studies related to 1) neuronal development, 2) neurobiology, 3) neurobiochemistry, and 4) neurotoxicity.

for PC-12 nuclear extractExtract Origin

Rat adrenal gland; pheochromocytoma (kidney tumor)

for PC-12 nuclear extractExtract Composition

PC-12 nuclear extract is supplied in Dilution Buffer (20 mM Hepes pH 7.5, 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM β-glycerophosphate, 1 mM DTT and protease inhibitors).

for PC-12 nuclear extractQuality Control

Extracts have been quality control tested by Western blot and the TransAM® Transcription Factor ELISA Kit.

for PC-12 nuclear extractStorage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for PC-12 nuclear extractGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

PC-12 nuclear extract