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Cell, Acid & Tissue Extracts

Raji nuclear extract

Catalog No: 36023 Format: 200 µg $165 Buy Now

for Raji nuclear extractContents

2 x 100 µg of Raji nuclear extract at 2.5 µg/µl.

for Raji nuclear extractBackground

Raji nuclear extract was prepared from cell cultures of the Raji Burkitt's Lymphoma cell line. This lymphoblastoid cell line was derived from a Nigerian patient with Burkitt's lymphoma and is the first continuous cell line of hematopoietic origin. These cells are most commonly used in studies related to hematology and related cancers, such as leukemia and lymphoma. Raji cells are a line of Epstein-Barr virus (EBV)-transformed lymphocytes and thus harbor latent EBV genomes and various chromosomal translocations, making them an ideal source for material for cytogenetic analysis. In addition, these cells express numerous surface Fc receptors and are routinely used for detection and analysis of immune complexes.

for Raji nuclear extractApplication Notes

Raji nuclear extract is specifically recommended for studies related to 1) hematology, 2) leukemia and lymphoma, 3) cytogenetics, and 4) immunology.

for Raji nuclear extractExtract Origin

Human B Lymphoblast (Burkitt's)

for Raji nuclear extractExtract Composition

Raji nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

for Raji nuclear extractQuality Control

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

 
Figure 1: Monitoring FKHR activity with the TransAM FKHR Kit.

Figure 1: Monitoring FKHR activity with the TransAM FKHR Kit.
Different amounts of nuclear extracts from untreated Raji (purple bars) and HeLa (copper bars) cells are tested for FKHR activity using the TransAM FKHR Kit.

Figure 1: NFκB family profiling of DNA binding activation in various cell lines.

Figure 1: NFκB family profiling of DNA binding activation in various cell lines.
Nuclear extracts prepared from HeLa, HeLa treated with TNF-α, Jurkat, Jurkat treated with PMA and calcium ionophore, and Raji cells were assayed at 10 µg/well for p65, p50, c-Rel, p52 and RelB activity using the TransAM NFκB Family Kit.

for Raji nuclear extractStorage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for Raji nuclear extractGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Raji nuclear extract
 

Data Thumbnails

Figure 1: Monitoring FKHR activity with the TransAM FKHR Kit.

Assay of FKHR activation using the TransAM FKHR Kit.
(Click image to enlarge and see details.)

Figure 1: NFκB family profiling of DNA binding activation in various cell lines.

Assay of NFκB family activation using the TransAM NFκB Family Kit.
(Click image to enlarge and see details.)