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Cell, Acid & Tissue Extracts

Saos-2 nuclear extract

Catalog No: 36025 Format: 200 µg $165 Buy Now

for Saos-2 nuclear extractContents

2 x 100 µg of Saos-2 nuclear extract at 2.5 µg/µl.

for Saos-2 nuclear extractBackground

Saos-2 nuclear extract was prepared from cell cultures of the human sarcoma osteogenic Saos-2 cell line. This non-transformed cell line was derived from the primary osteosarcoma of an 11-year old Caucasian female patient. Saos-2 cells exhibit many characteristic osteoblastic properties, including high levels of alkaline phosphatase expression, parathyroid hormone-mediated induction of adenylate cyclase production, and production and secretion of type I collagen and mineralized matrix. Therefore, they are commonly used to study osteoblastic phenotype-related cellular functions and products and in research related to late-stage osteoblastic differentiation.

for Saos-2 nuclear extractApplication Notes

Saos-2 nuclear extract is specifically recommended for studies related to osteoblasts and osteoblastic differentiation.

for Saos-2 nuclear extractExtract Origin

Human osteosarcoma cells

for Saos-2 nuclear extractExtract Composition

Saos-2 nuclear extract is supplied in 20 mM Hepes pH 7.5, 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM β-glycerophosphate, 1 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay.

for Saos-2 nuclear extractQuality Control

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

for Saos-2 nuclear extractStorage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for Saos-2 nuclear extractGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Saos-2 nuclear extract