ZR-75-1 nuclear extract

for ZR-75-1 nuclear extractContents

200 µg of ZR-75-1 nuclear extract at 5.4 µg/µl.

for ZR-75-1 nuclear extractBackground

ZR-75-1 nuclear extract was prepared from cell cultures of the human breast carcinoma ZR-75-1 cell line. The ZR-75-1 cell line was derived from a malignant effusion in a 63-year old Caucasian female patient with infiltrating ductal carcinoma. These epithelial-like cells express both wild type and mutant receptors to estradiol, receptors to progesterone, as well as other steroids. In addition, high levels of mRNA expression of the human tumor antigen MUC-1 has been observed in ZR-75-1 cells. The ZR-75-1 cell line is commonly used in research as in vitro model for studies of breast cancer tumorigenicity and related drug therapies, such as tamoxifen.

for ZR-75-1 nuclear extractApplication Notes

ZR-75-1 nuclear extract is specifically recommended for in studies related to breast cancer and associated drug therapies.

for ZR-75-1 nuclear extractExtract Origin

Human breast carcinoma

for ZR-75-1 nuclear extractExtract Composition

ZR-75-1 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl₂, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

for ZR-75-1 nuclear extractQuality Control

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

for ZR-75-1 nuclear extractStorage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for ZR-75-1 nuclear extractGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.