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Cell, Acid & Tissue Extracts

Caco-2 nuclear extract

Catalog No: 36035 Format: 200 µg $165 Buy Now

for Caco-2 nuclear extractContents

200 µg of Caco-2 nuclear extract at 7.11 µg/µl.

for Caco-2 nuclear extractBackground

Caco-2 nuclear extract was prepared from cell cultures of the human colorectal adenocarcinoma Caco-2 cell line. The Caco-2 cell line was originally derived from a colon carcinoma and functionally demonstrates properties resembling enterocytes of the small intestine. When confluent, these cells form a polarized epithelial monolayer with properties similar to those of the mucosal lining of the small intestine. In research, Caco-2 cells are most commonly as in vitro models of the small intestinal mucosa to study the physiological and biochemical properties of intestinal permeability, adsorption, transport and metabolism of ions, small molecules, and drug therapies.

for Caco-2 nuclear extractApplication Notes

Caco-2 nuclear extract is specifically recommended for analysis of the biophysical and biochemical properties associated with small intestinal mucosa.

for Caco-2 nuclear extractExtract Origin

Human Colorectal Adenocarcinoma

for Caco-2 nuclear extractExtract Composition

Caco-2 cells are grown to 90% confluence before harvesting' the nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

for Caco-2 nuclear extractQuality Control

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

for Caco-2 nuclear extractStorage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for Caco-2 nuclear extractGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Caco-2 nuclear extract