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Cell, Acid & Tissue Extracts

Jurkat nuclear extract (Heat Shock)

Catalog No: 36069 Format: 200 µg $165 Buy Now

for Jurkat nuclear extract (Heat Shock)Contents

200 µg of Jurkat nuclear extract (Heat Shock treated) at 3.97 µg/µl.

for Jurkat nuclear extract (Heat Shock)Background

Jurkat nuclear extract (Heat Shock) was prepared from cell cultures of the Jurkat human CD4(+) T cell lymphoblast-like cell line. The Jurkat cell line was established from the propagation of peripheral blood cells of a 14 year old boy with T cell leukemia. These cells are primarily used in research as a T cell model system, particularly in studies of T cell signaling, T cell leukemia, and response to viral infection. Jurkat cells are frequently used in HIV-related research to examine the mechanisms governing T cell-mediated immunity in response to HIV infection. Stimulation of these cells with phorbol esters and either lectins or monoclonal antibodies leads to activation of T cell antigen receptors and induction of IL-2 expression. Because these cells can produce IL-2 in response to stimulation, they are frequently used in research to study the regulation of cytokine expression and differentiation. However, the primary use of Jurkat cells is to study the response of various cancers to radiation or drug treatments.

Heat shock treatment of Jurkat cells induces a stress response that subsequently leads to apoptosis. Heat shock treated Jurkat cell lines are frequently used to study the physiological response of cells to heat stress and the processes leading to stress-induced apoptosis.

for Jurkat nuclear extract (Heat Shock)Application Notes

Jurkat nuclear extract (Heat Shock) is specifically recommended for use in studies related to 1) T cell-mediated immunity and response to viral infection (e.g. HIV), 2) cytokine induction and cell differentiation, 3) cellular response to cancer therapies, and 4) heat stress and stress-induced apoptosis.

for Jurkat nuclear extract (Heat Shock)Extract Origin

Human T lymphoblast; acute T cell leukemia

for Jurkat nuclear extract (Heat Shock)Extract Composition

Jurkat nuclear extract (Heat Shock) is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). The nuclear extract was prepared from the heat shock-treated Jurkat human T cell leukemia cell line (clone E6-1). Cells were cultured in medium that was heated at 45°C for 45 minutes immediately prior to harvesting.

for Jurkat nuclear extract (Heat Shock)Quality Control

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

for Jurkat nuclear extract (Heat Shock)Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for Jurkat nuclear extract (Heat Shock)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Jurkat nuclear extract (Heat Shock)