K-562 nuclear extract (TPA stimulated)

for K-562 nuclear extract (TPA stimulated)Contents

2 x 100 µg of K-562 nuclear extract (TPA stimulated) at 2.5 µg/µl.

for K-562 nuclear extract (TPA stimulated)Background

K-562 nuclear extract (TPA stimulated) was prepared from a cell culture of the K-562 human myelogenous leukaemia cell line. The K-562 cell line was originally derived from immortalized erythroleukaemia cells obtained from a patient with a Philadelphia chromosome-positive chronic myeloid leukemia in blast crisis. These pluripotent cells are widely applicable in research due to their ability to spontaneously differentiate into erythrocyte, granulocyte and monocyte progenitors. K-562 cells are also frequently utilized in in vitro natural killer (NK) cell assays as highly sensitive targets since they lack the expression of MHC class I molecules required for protection against NK activity.

Treatment of K-562 cells with the potent tumor-promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) leads to various biological changes that mimic those observed in cells transformed by chemical carcinogens or viruses. Tumor promoters such as TPA are thought to play a significant role in the critical first step in multistage tumorigenesis known as tumor conversion. Treatment of K-562 cells with TPA leads to induction of megakaryocytic differentiation and decreased susceptibility to NK activity. Thus, TPA stimulated K-562 cells are frequently utilized in cancer research as a model system for studying the radiomimetic and tumor-promoting effects of TPA on the differentiation stage of human malignant cells.

for K-562 nuclear extract (TPA stimulated)Application Notes

K-562 nuclear extract is recommended for use in studies related to 1) differentiation, 2) immunology, and 3) cancer. K-562 nuclear extract (TPA stimulated) is specifically recommended for analysis of the mechanisms underlying tumorigenesis or TPA-mediated tumor conversion and its associated biochemical responses, including increased membrane metabolism, induction of protein biosynthesis, and alterations in the cell cycle.

for K-562 nuclear extract (TPA stimulated)Extract Origin

Human lymphoblast bone marrow; chronic myelogenous leukemia (CML)

for K-562 nuclear extract (TPA stimulated)Extract Composition

K-562 nuclear extract (TPA stimulated) is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl₂, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). Cells were cultured in medium supplemented with 50 ng/ml TPA (phorbol, 12-myristate, 13 acetate) for two hours at 37°C immediately prior to harvesting.

for K-562 nuclear extract (TPA stimulated)Quality Control

Extracts have been quality control tested by Western blot and TransAM^® Transcription Factor ELISAs.

for K-562 nuclear extract (TPA stimulated)Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for K-562 nuclear extract (TPA stimulated)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.