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Cell, Acid & Tissue Extracts

Nb2 nuclear extract

Catalog No: 36105 Format: 200 µg $165 Buy Now

for Nb2 nuclear extractContents

2 x 100 µg of Nb2 nuclear extract at 2.5 µg/µl.

for Nb2 nuclear extractBackground

Nb2 nuclear extract was prepared from cell cultures of the rat lymphoma Nb2 nuclear cell line. The Nb2 cell line was derived from the lymphoma transplant in the thymus of a noble (Nb) strain male rat following prolonged estrogen treatment. Nb2 lymphoma cells are of pre-T cell origin and express the alpha and beta chains of the T cell receptor. However, they lack expression of class II MHC antigens. These cells are dependent on lactogens for mitogenic activity, which can also be stimulated by cytokines and human growth hormone and its analogs. Nb2 cells are most commonly used in studies of lactogen-induced mitogenesis and as a model for tumor progression of T cell cancers.

for Nb2 nuclear extractApplication Notes

Nb2 nuclear extract is specifically recommended for studies related to lactogen-induced mitogenesis and T cell cancers.

for Nb2 nuclear extractExtract Origin

Rat lymphocytes

for Nb2 nuclear extractExtract Composition

Nb2 nuclear extract derived from rat lymphocytes was collected in Lysis Buffer. Lysis Buffer consists of 20 mM Hepes pH 7.9, 100 mM KCl, 20% glycerol, 1 mM MgCl2, 0.5 mM DTT and 0.5 mM PMSF. The protein content has been determined by a Bradford-based assay.

for Nb2 nuclear extractQuality Control

Each lot has been tested for STAT activation by using TransAM® STAT Family Kits.

for Nb2 nuclear extractStorage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for Nb2 nuclear extractGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Nb2 nuclear extract