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Cell, Acid & Tissue Extracts

HeLa nuclear extract (Anisomycin treated)

Catalog No: 36111 Format: 200 µg $165 Buy Now

for HeLa nuclear extract (Anisomycin treated)Contents

2 x 100 µg of HeLa nuclear extract (Anisomycin treated) at 2.5 µg/µl.

for HeLa nuclear extract (Anisomycin treated)Background

HeLa nuclear extract (Anisomycin treated) was prepared from a cell culture of the HeLa human epithelial carcinoma cell line that was treated with anisomycin, an antibiotic and protein synthesis inhibitor. Anisomycin is also a potent inhibitor of the p38/JNK MAPK pathway.

The HeLa cell line was originally derived from cells obtained in 1951 from Henrietta Lacks, a cervical cancer patient who eventually died from her cancer. The HeLa cell line is one of the oldest and most commonly utilized immortal cell lines in scientific research. These cells are highly prolific and easily sustainable, allowing for flexibility in their application in various fields of scientific research, including cancer biology, virology, and human disease. To date, over 60,000 publications across various disciplines have referenced the use of HeLa cells.

for HeLa nuclear extract (Anisomycin treated)Application Notes

HeLa nuclear extract (Anisomycin treated) is specifically recommended for analysis of protein synthesis and the p38/JNK MAPK pathway.

for HeLa nuclear extract (Anisomycin treated)Extract Origin

Human Cervix Adenocarcinoma

for HeLa nuclear extract (Anisomycin treated)Extract Composition

HeLa nuclear extract is supplied in Dilution Buffer (20 mM Hepes pH 7.5, 400 mM NaCl, 20% glycerol, 0.1 mM EDTA, 10 mM NaF, 10 µM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM β-glycerophosphate, 1 mM DTT and protease inhibitors). Cells were cultured in medium supplemented with 5 µM Anisomycin for 1 hour at 37°C immediately prior to harvesting.

for HeLa nuclear extract (Anisomycin treated)Quality Control

Each lot has been tested for ATF-2 activation by using TransAM® Kits.

 
Figure 1: Monitoring ATF-2 activation with the TransAM ATF-2 Kit.

Figure 1: Monitoring ATF-2 activation with the TransAM ATF-2 Kit.
Increasing amounts of nuclear extract from unstimulated HeLa and Anisomycin-stimulated HeLa cells are tested for ATF-2 activation using the TransAM ATF-2 Kit.

for HeLa nuclear extract (Anisomycin treated)Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for HeLa nuclear extract (Anisomycin treated)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

HeLa nuclear extract (Anisomycin treated)
 

Data Thumbnails

Figure 1: Monitoring ATF-2 activation with the TransAM ATF-2 Kit.

Assay of ATF-2 activation using the TransAM ATF-2 Kit.
(Click image to enlarge and see details.)