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Cell, Acid & Tissue Extracts

Hep G2 whole-cell extract (IL-6 stimulated, 10 ng/ml)

Catalog No: 36413 Format: 200 µg $165 Buy Now

for Hep G2 whole-cell extract (IL-6 stimulated, 10 ng/ml)Contents

200 µg of Hep G2 whole-cell extract (IL-6 stimulated) at 5.0 µg/µl.

for Hep G2 whole-cell extract (IL-6 stimulated, 10 ng/ml)Background

Hep G2 whole-cell extract (IL-6 stimulated, 10 ng/ml) was prepared from cell cultures of the human hepatocellular liver carcinoma Hep G2 cell line. These cells are epithelial in morphology and can be induced to exhibit polarization under appropriate culture conditions. Hep G2 cells are most commonly used for studies of hepatocyte function. Because of their ability to differentiate into polarized epithelium, these cells are routinely utilized to investigate intracellular protein trafficking, particularly in relation to human liver diseases. Hep G2 is the most commonly used cell line in studies pertaining to the regulation of hepatic protein synthesis, particularly the synthesis of acute phase proteins after the onset of a systemic inflammatory response. In addition, these cells are important for studies related to liver metabolism, liver cancers, liver regeneration, liver cytotoxicity, apoptosis and as a model for hepatitis B virus (HBV) viral etiology.

In response to injury, disease or infection, interleukin-6 (IL-6) is a cytokine that is rapidly and transiently expressed as a host defense mechanism. IL-6 has various biological activities, most of which are associated with acute phase immune and inflammatory responses. In the liver, IL-6 is major regulator of acute phase protein synthesis in response to inflammation. Studies have demonstrated that constitutive IL-6 has deleterious effects on the liver and may be involved in the onset of liver disease, pathogenesis and cancer.

for Hep G2 whole-cell extract (IL-6 stimulated, 10 ng/ml)Application Notes

Hep G2 whole-cell extract is specifically recommended for studies associated with inflammation of the liver and related to 1) human liver diseases, cancers and drug therapies, 2) HBV etiology, 3) signaling pathways, and 4) apoptosis.

for Hep G2 whole-cell extract (IL-6 stimulated, 10 ng/ml)Extract Origin

Human Liver (hepatocellular carcinoma)

for Hep G2 whole-cell extract (IL-6 stimulated, 10 ng/ml)Extract Composition

Hep G2 whole-cell extract (IL-6 stimulated, 10 ng/ml) is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). Cells were cultured in medium supplemented with 10 ng/ml IL-6 (interleukin 6) for 15 minutes at 37°C immediately prior to harvesting.

for Hep G2 whole-cell extract (IL-6 stimulated, 10 ng/ml)Quality Control

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

for Hep G2 whole-cell extract (IL-6 stimulated, 10 ng/ml)Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

for Hep G2 whole-cell extract (IL-6 stimulated, 10 ng/ml)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

 

Technical Data Sheet

Hep G2 whole-cell extract (IL-6 stimulated, 10 ng/ml)